Human IL-3R alpha /CD123 APC-conjugated Antibody Summary
Lys20-Arg305, predicted
Accession # P26951
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑3 R alpha /CD123 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA-DR PE-conjugated Monoclonal Antibody (Catalog # FAB4869P) and either (A) Mouse Anti-Human IL-3 Ra/CD123 APC-conjugated Monoclonal Antibody (Catalog # FAB301A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-3R alpha
IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). IL-3 exerts its activity through binding to a specific cell surface receptor known as IL-3 R. IL-3 R is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (CD123) and a 120-140 kDa IL-3 R beta subunit (CD131) (3, 4). IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL‑3 R beta chain has a very large cytoplasmic domain. The IL‑3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes (5).
- Moore, M.A.S. et al. (1991) Blood 72:944.
- Warren, D.J. et al. (1988) J. Immunol. 140:94.
- Plant M. et al. (1989) Nature 339:150.
- Budel, L.M. et al. (1990) Blood 75:1439.
- Schrader, J.W. et al. (1988) In Interleukin-3: The Panspecific hemopoietin (ed. J.W. Schrader), Academic Press, San Diego, CA.
Product Datasheets
Citation for Human IL-3R alpha /CD123 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Subthreshold IKK activation modulates the effector functions of primary mast cells and allows specific targeting of transformed mast cells.
Authors: Drube S, Weber F, Loschinski R, Beyer M, Rothe M, Rabenhorst A, Gopfert C, Meininger I, Diamanti M, Stegner D, Hafner N, Bottcher M, Reinecke K, Herdegen T, Greten F, Nieswandt B, Hartmann K, Kramer O, Kamradt T
Oncotarget, 2015-03-10;6(7):5354-68.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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