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Human IL-3R alpha /CD123 PerCP-conjugated Antibody

Catalog #: FAB301C
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FAB301C
Detection of IL‑3 R alpha /CD123 in Human Blood Lymphocytes by Flow Cytometry.
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Human IL-3R alpha /CD123 PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑3 R alpha /CD123 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 32703
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑3 R alpha /CD123
Lys20-Arg305, predicted
Accession # P26951
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL‑3 Ra/CD123 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of IL‑3 R alpha /CD123 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA-DR PE-conjugated Monoclonal Antibody (Catalog # FAB4869P) and either (A) Mouse Anti-Human IL-3 Ra/CD123 PerCP-conjugated Monoclonal Antibody (Catalog # FAB301C) or (B) Mouse IgG1PerCP Isotype Control (Catalog # IC002C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-3R alpha

IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). IL-3 exerts its activity through binding to a specific cell surface receptor known as IL-3 R. IL-3 R is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (CD123) and a 120-140 kDa IL-3 R beta subunit (CD131) (3, 4). IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL‑3 R beta chain has a very large cytoplasmic domain. The IL‑3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes (5).

References
  1. Moore, M.A.S. et al. (1991) Blood 72:944.
  2. Warren, D.J. et al. (1988) J. Immunol. 140:94.
  3. Plant M. et al. (1989) Nature 339:150.
  4. Budel, L.M. et al. (1990) Blood 75:1439.
  5. Schrader, J.W. et al. (1988) In Interleukin-3: The Panspecific hemopoietin (ed. J.W. Schrader), Academic Press, San Diego, CA.
Long Name
Interleukin 3 Receptor alpha
Entrez Gene IDs
3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)
Alternate Names
CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174

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Citation for Human IL-3R alpha /CD123 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A role for IL-25 and IL-33-driven type-2 innate lymphoid cells in atopic dermatitis.
    Authors: Salimi M, Barlow J, Saunders S, Xue L, Gutowska-Owsiak D, Wang X, Huang L, Johnson D, Scanlon S, McKenzie A, Fallon P, Ogg G
    J Exp Med, 2013-12-09;210(13):2939-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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