Human IL-7 Antibody Summary
Accession # P13232
Applications
Human IL-7 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody. Recombinant Human IL-7 (Catalog # 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-7 Monoclonal Antibody (Catalog # MAB207). The ND50 is typically 0.4-0.8 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-7
IL-7, previously known as pre-B cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4+ and CD8+ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.
Human IL-7 cDNA encodes a precursor protein of 177 amino residues containing a 25 amino acid residue signal peptide. Mouse IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity.
IL-7 bioactivites are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells, and bone marrow cells.
Product Datasheets
Citations for Human IL-7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Macrophages are the primary effector cells in IL-7-induced arthritis
Authors: SJ Kim, HJ Chang, MV Volin, S Umar, K Van Raemdo, A Chevalier, K Palasiewic, JW Christman, S Volkov, S Arami, M Maz, A Mehta, RK Zomorrodi, DA Fox, N Sweiss, S Shahrara
Cell. Mol. Immunol., 2019-06-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization -
Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions
Authors: T Murakami, J Kim, Y Li, GE Green, A Shikanov, A Ono
Nat Commun, 2018-06-22;9(1):2436.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
Sci Rep, 2016-09-02;6(0):32320.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization -
Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
J Biol Chem, 2015-07-16;290(37):22338-51.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The novel role of IL-7 ligation to IL-7 receptor in myeloid cells of rheumatoid arthritis and collagen-induced arthritis.
Authors: Chen Z, Kim S, Chamberlain N, Pickens S, Volin M, Volkov S, Arami S, Christman J, Prabhakar B, Swedler W, Mehta A, Sweiss N, Shahrara S
J Immunol, 2013-04-19;190(10):5256-66.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
Int. J. Cancer, 2009-09-01;125(5):1092-101.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Ultrasensitive flow-based immunoassays using single-molecule counting.
Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clin. Chem., 2007-09-21;53(11):1990-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood, 2007-02-08;109(10):4272-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Interleukin-7 improves CD4 T-cell reconstitution after autologous CD34 cell transplantation in monkeys.
Authors: Storek J, Gillespy T, Lu H, Joseph A, Dawson MA, Gough M, Morris J, Hackman RC, Horn PA, Sale GE, Andrews RG, Maloney DG, Kiem HP
Blood, 2003-01-23;101(10):4209-18.
Species: Primate - Papio cynocephalus (Yellow Baboon)
Sample Types: Whole Cells
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The antibody was used in an ELISA application. The R&D antibody AF-207 was used as the detection. IL-7 in human samples was measured.
