Human IL-8/CXCL8 Biotinylated Antibody Summary
Applications
Human IL-8/CXCL8 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
IL-8/CXCL8 in Human PBMCs. IL-8/CXCL8 was detected in immersion fixed PMA-, ionomycin- and monensin-activated human peripheral blood mononuclear cells (PBMCs) using Human IL-8/CXCL8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: IL-8/CXCL8
CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc. CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.
Product Datasheets
Citations for Human IL-8/CXCL8 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Effect of teriparatide on drug treatment of tuberculous spondylitis: an experimental study
Authors: S Lee, YJ Seo, JY Choi, X Che, HJ Kim, SY Eum, MS Hong, SK Lee, DC Cho
Scientific Reports, 2022-12-15;12(1):21667.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Detection -
Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case
Authors: S Abouelasra, M De Bondt, N Berghmans, M Gouwy, VLS de Oliveir, SC Oliveira, FA Amaral, P Proost, J Van Damme, S Struyf, M De Buck
Mediators Inflamm., 2020-07-06;2020(0):6087109.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA -
Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease
Authors: J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard
PLoS ONE, 2017-09-07;12(9):e0184039.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection) -
Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease
Authors: MM White, P Geraghty, E Hayes, S Cox, W Leitch, B Alfawaz, GM Lavelle, OJ McElvaney, R Flannery, J Keenan, P Meleady, M Henry, M Clynes, C Gunaratnam, NG McElvaney, EP Reeves
EBioMedicine, 2017-08-16;23(0):173-184.
Species: Human
Sample Types: Plasma
Applications: ELISA Development (Detection) -
Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
Authors: Voltolini C, Battersby S, Novembri R, Torricelli M, Severi F, Petraglia F, Norman J
Endocrinology, 2014-11-26;156(2):670-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection) -
Daily exposure to dust alters innate immunity.
Authors: Sahlander K, Larsson K, Palmberg L
PLoS ONE, 2012-02-15;7(2):e31646.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Protection from RNA and DNA viruses by IL-32.
Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
J. Immunol., 2011-02-23;186(7):4110-8.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Electrochemiluminescent Assay -
Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
J. Immunol., 2010-10-22;185(11):7047-56.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009-06-27;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development -
Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
Am. J. Physiol. Lung Cell Mol. Physiol., 2009-06-12;297(2):L388-400.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
Authors: Osei-Kumah A, Smith R, Clifton VL
Cytokine, 2008-07-17;43(2):187-93.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Endogenous IL-32 controls cytokine and HIV-1 production.
Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
J. Immunol., 2008-07-01;181(1):557-65.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Electrochemiluminescent Assay -
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clin. Chem., 2007-11-01;53(11):2010-2.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007-10-22;329(1):214-8.
Species: Human
Sample Types: Serum
Applications: Luminex Development -
Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
Clin. Cancer Res., 2007-10-01;13(19):5719-28.
Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development -
Ultrasensitive flow-based immunoassays using single-molecule counting.
Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clin. Chem., 2007-09-21;53(11):1990-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Tumour necrosis factor-alpha (TNF-alpha) increases nuclear factor kappaB (NFkappaB) activity in and interleukin-8 (IL-8) release from bovine mammary epithelial cells.
Authors: Fitzgerald DC, Meade KG, McEvoy AN, Lillis L, Murphy EP, Machugh DE, Baird AW
Vet. Immunol. Immunopathol., 2007-01-09;116(1):59-68.
Species: Bovine
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
Infect. Immun., 2006-09-18;74(12):6730-8.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clin. Immunol., 2006-06-16;120(2):220-31.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
PAPP-A and osteoprotegerin, together with interleukin-8 and RANTES, are elevated in the peritoneal fluid of women with endometriosis.
Authors: Bersinger NA, von Roten S, Wunder DM, Raio L, Dreher E, Mueller MD
Am. J. Obstet. Gynecol., 2006-04-25;195(1):103-8.
Species: Human
Sample Types: Peritoneal Fluid
Applications: ELISA Development -
Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
Authors: Ramachandran R, Morice AH, Compton SJ
Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):133-41.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
Authors: Edwards MR, Johnson MW, Johnston SL
Am. J. Respir. Cell Mol. Biol., 2006-01-19;34(5):616-24.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Increased ENA-78 in the follicular fluid of patients with endometriosis.
Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
Acta Obstet Gynecol Scand, 2006-01-01;85(3):336-42.
Species: Human
Sample Types: Follicular Fluid
Applications: ELISA Development -
Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery.
Authors: Holst RM, Mattsby-Baltzer I, Wennerholm UB, Hagberg H, Jacobsson B
Acta Obstet Gynecol Scand, 2005-06-01;84(6):551-7.
Species: Human
Sample Types: Amniotic Fluid
Applications: ELISA Development -
17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
Blood, 2004-05-13;104(5):1404-10.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Authors: Copeland S, Siddiqui J, Remick D
J. Immunol. Methods, 2004-01-01;284(1):99-106.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Array Development -
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
J. Immunol., 2002-12-01;169(11):6515-21.
Species: Human
Sample Types: BALF
Applications: ELISA Development -
Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
J. Immunol., 2002-11-01;169(9):5244-51.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Metabolic replacement of kidney function in uremic animals with a bioartificial kidney containing human cells.
Authors: Humes HD, Fissell WH, Weitzel WF, Buffington DA, Westover AJ, MacKay SM, Gutierrez JM
Am. J. Kidney Dis., 2002-05-01;39(5):1078-87.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development -
Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
Authors: Hirst SJ, Hallsworth MP, Peng Q
Am. J. Respir. Crit. Care Med., 2002-04-15;165(8):1161-71.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
Immunity, 2001-06-01;14(6):705-14.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Chemoattractant factors in breast milk from allergic and nonallergic mothers.
Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
Pediatr. Res., 2000-05-01;47(5):592-7.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Peritonitis in the baboon: a primate model which stimulates human sepsis.
Authors: Kinasewitz GT, 2016, Chang AC, Peer GT, Hinshaw LB, Taylor FB
2000-02-01;13(2):100-9.
Species: Primate - Papio cynocephalus (Yellow Baboon)
Sample Types: Plasma
Applications: ELISA Development
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