Human ILT1/CD85h APC-conjugated Antibody

Catalog #: FAB6364A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB6364A

Detection of ILT1/CD85h in Human Blood Monocytes by Flow Cytometry.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ILT1/CD85h APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ILT1/CD85h in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT2, 3, 4, 5, rhLIR6, or rhLIR8 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 600007

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ILT1/CD85h
Pro17-Ser437
Accession # Q8N149

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of ILT1/CD85h in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human ILT1/CD85h APC-conjugated Monoclonal Antibody (Catalog # FAB6364A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRA2/CD85h/ILT1

Immune-Like Transcript 1 (ILT1), also known as Leukocyte Immunoglobulin-like Receptor 7 (LIR-7), Leukocyte Immunoglobulin-like Receptor subfamily A member 2 (LILRA2), and CD85h, is a 51 kDa (unglycosylated) type I transmembrane glycoprotein and member of the leukocyte immunoglobulin-like receptor family. Human ILT1 is synthesized as a 483 amino acid (aa) precursor that contains a 23 aa signal sequence, a 426 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic region. The ECD contains four Ig-like C2-type domains and seven potential sites for N-linked glycosylation. A splice variant produces a second isoform that has a one aa substitution for aa 419-436 in the longer form. There are no murine orthologs for human ILT1. ILT1 may act as a receptor for class I MHC antigens.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 2

Entrez Gene IDs

11027 (Human)

Alternate Names

CD85 antigen-like family member H; CD85h antigen; CD85h; ILT1; ILT-1; ILT1CD85H; Leukocyte immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily A member 2 soluble; leukocyte immunoglobulin-like receptor subfamily A member 2; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2; LILRA2; LIR7; LIR7LIR-7Immunoglobulin-like transcript 1