Human ILT11/LILRA5 PE-conjugated Antibody

Catalog #: FAB6754P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB6754P

Detection of ILT11/LILRA5 in Human Blood Monocytes by Flow Cytometry.

1 Image

All LILRA5/CD85f Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ILT11/LILRA5 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ILT11/LILRA5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ILT11/LILRA5, recombinant rat (rr) ILT11/LILRA5, rrLILRC2, recombinant human (rh) ILT4/CD85d, rhILT5/CD85a, or rhILT3/CD85k is observed.

Source

Monoclonal Mouse IgG_2A Clone # 711828

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ILT11/LILRA5
Gly42-Arg268
Accession # A6NI73

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of ILT11/LILRA5 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3832C) and either (A) Mouse Anti-Human ILT11/LILRA5 PE-conjugated Monoclonal Antibody (Catalog # FAB6754P) or (B) Mouse IgG_2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRA5/CD85f

ILT11 (also leukocyte immunoglobulin-like receptor 9 (LIR-9), CD85f, and LILRA5) is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors. It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-a and IL-1b. Mature human ILT11 is a 258 amino acid (aa) type I transmembrane glycoprotein. It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains (aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail. ILT11 has three potential splice forms. One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299. The other two splice forms are analogous to the above membrane and soluble forms, but demonstrate signal sequence cleavage further downstream after Ala51. Over aa 41-268, human ILT11 shares 58% aa identity with mouse ILT11.

Long Name

Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5

Entrez Gene IDs

353514 (Human); 232801 (Mouse); 691533 (Rat)

Alternate Names

CD85 antigen-like family member F; CD85; CD85f antigen; CD85f; ILT11; ILT-11; ILT11CD85f; Immunoglobulin-like transcript 11; leukocyte Ig-like receptor 9; Leukocyte immunoglobulin-like receptor 9; leukocyte immunoglobulin-like receptor subfamily A member 5 soluble; leukocyte immunoglobulin-like receptor subfamily A member 5; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRA5; LILRB7; LIR9; LIR-9; LIR9immunoglobulin-like transcript 11 protein; member 7