Human Integrin alpha 4/CD49d Antibody

Catalog #: BBA37
11 Citations Datasheet / COA / SDS

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BBA37

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Citations (11)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin alpha 4/CD49d Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha 4 subunit (Needham, L.A. et al. (1994) Cell Adhesion and Communication 2:87).

Source

Monoclonal Mouse IgG₁ Clone # 2B4

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

U937 human histiocytic lymphoma cell line

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Adhesion Blockade

The adhesion of U937 human histiocytic lymphoma cells (5 x 10⁴ cells/well) to immobilized recombinant human VCAM-1 (10 μg/mL, 100 µL/well) was maximally inhibited (80 - 100%) by 5 μg/mL of the antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4/CD49d

Integrin alpha 4, also known as CD49d, is a transmembrane protein that forms heterodimers with beta 1 or beta 7 integrins. These complexes contribute to the trafficking of many hematopoietic cell types. Integrins alpha 4 beta 1 (also known as VLA-4) and alpha 4 beta 7 (LPAM-1) are receptors for fibronectin, VCAM and MAdCAM-1.

Entrez Gene IDs

3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)

Alternate Names

269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Integrin alpha 4/CD49d Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to ?4?7
Authors: S Lertjuthap, C Cicala, D Van Ryk, M Liu, J Yolitz, D Wei, F Nawaz, A Doyle, B Horowitch, C Park, S Lu, Y Lou, S Wang, R Pan, X Jiang, F Villinger, SN Byrareddy, PJ Santangelo, L Morris, CK Wibmer, K Biris, RD Mason, J Gorman, J Hiatt, E Martinelli, M Roederer, D Fujikawa, G Gorini, G Franchini, A Arakelyan, AA Ansari, K Pattanapan, XP Kong, AS Fauci, J Arthos
PLoS Pathog., 2018-08-28;14(8):e1007278.
Species: Human
Sample Types: Recombinant Protein
Applications: Neutralization
Humanized mouse model of Rasmussen's encephalitis supports the immune-mediated hypothesis
Authors: H Kebir, L Carmant, F Fontaine, K Béland, CM Bosoi, NT Sanon, JI Alvarez, S Desgent, CL Pittet, D Hébert, MJ Langlois, RM Rébillard, DK Nguyen, C Cieuta-Wal, GL Holmes, HP Goodkin, JR Mytinger, MB Connolly, A Prat, E Haddad
J. Clin. Invest., 2018-04-09;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis
Authors: T Kobori, S Hamasaki, A Kitaura, Y Yamazaki, T Nishinaka, A Niwa, S Nakao, H Wake, S Mori, T Yoshino, M Nishibori, H Takahashi
Front Immunol, 2018-03-06;9(0):334.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC, Neutralization
Bilirubin Prevents Atherosclerotic Lesion Formation in Low-Density Lipoprotein Receptor-Deficient Mice by Inhibiting Endothelial VCAM-1 and ICAM-1 Signaling
Authors: ME Vogel, G Idelman, ES Konaniah, SD Zucker
J Am Heart Assoc, 2017-04-01;6(4):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells.
Authors: Yoon S, Lee I, Zhang X, Zapata M, Choi Y
FEBS Open Bio, 2014-04-13;4(0):370-6.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor beta (TGFbeta)-, epidermal growth factor (EGF)-, and nuclear factor kappaB (NFkappaB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion.
Authors: Kang I, Yoon D, Braun K, Wight T
J Biol Chem, 2014-04-09;289(22):15393-404.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-beta1 production and FcRL4 expression.
Authors: Jelicic K, Cimbro R, Nawaz F, Huang D, Zheng X, Yang J, Lempicki R, Pascuccio M, Van Ryk D, Schwing C, Hiatt J, Okwara N, Wei D, Roby G, David A, Hwang I, Kehrl J, Arthos J, Cicala C, Fauci A
Nat Immunol, 2013-10-27;14(12):1256-65.
Species: Human
Sample Types: Whole Cells
Applications: Blocking
Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin.
Authors: Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schon MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH
Immunology, 2009-10-01;128(2):196-205.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
High pressure promotes monocyte adhesion to the vascular wall.
Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
Circ. Res., 2007-03-29;100(8):1226-33.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
Atherosclerosis, 2006-10-04;192(1):56-66.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Rho family small GTPases control migration of hematopoietic progenitor cells into multicellular spheroids of bone marrow stroma cells.
Authors: Bug G, Rossmanith T, Henschler R, Kunz-Schughart LA, Schroder B, Kampfmann M, Kreutz M, Hoelzer D, Ottmann OG
J. Leukoc. Biol., 2002-10-01;72(4):837-45.
Species: Human
Sample Types: Whole Cells
Applications: ICC