Home / Integrin alpha V beta 3 / Human Integrin  alpha V beta 3 Antibody

Human Integrin  alpha V beta 3 Antibody

Catalog #: MAB3050
19 Citations Datasheet / COA / SDS
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MAB3050
MAB3050-SP
Detection of Human Integrin alpha V beta 3 by Immunocytochemistry/Immunofluorescence
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Citations (19)
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Human Integrin  alpha V beta 3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha V beta 3.
Source
Monoclonal Mouse IgG1 Clone # 23C6
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human osteoclasts
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
HUVEC human umbilical vein endothelial cells
Immunohistochemistry
Davies, J. et al. (1989) J. Cell Biol. 109:1817.
 
Immunoprecipitation
Davies, J. et al. (1989) J. Cell Biol. 109:1817.
 
Adhesion Blockade
Horton, M.A. et al. (1991) Exp. Cell Res. 195:368. Bates, R.C. et al. (1998) Cell Adhes. Commun. 6:21.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry/ Immunofluorescence Detection of Human Integrin alpha V beta 3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Integrin alpha V beta 3 by Immunocytochemistry/Immunofluorescence Effects of AA and DHA on actin ring formation and VNR expression.Osteoclast differentiation was stimulated in CD14+ monocytes by the addition of 25 ng ml-1 M-CSF and 30 ng ml-1 RANKL as described in Materials and Methods. Vehicle (0.08% ethanol) or LCPUFAs (40 μorption (day 12–14) in mature osteoclasts. After 3 weeks of culture, cells were fixed and stained with Hoechst (blue) for nuclei, phalloidin (red) for actin rings, and anti-VNR antibody (green). The results are representative of three independent experiments conducted in triplicate. Scale bar = 200 μm. Diff Ocl—differentiating osteoclasts. Mat Ocl—mature osteoclasts. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25867515), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V beta 3

Integrin alpha V beta 3 together with alpha IIb beta 3, constitutes the only known beta 3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N-terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alpha V subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alpha V beta 3 (4, 10). The 962 aa human alpha V ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow  alpha V while the 685 aa human beta 3 ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta 3.

References
  1. Hynes, R. O. (2002) Cell 110:673.
  2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.
  3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
  4. Xiong, J. et al. (2001) Science 294:339.
  5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
  6. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
  7. Djaffar, I. et al. (1994) Biochem. J. 300:67.
  8. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
  9. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  10. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
  11. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.
  12. Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.
Entrez Gene IDs
3685 (Human)
Alternate Names
antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V beta 3; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; Vitronectin receptor subunit alpha; VNRADKFZp686A08142

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Citations for Human Integrin  alpha V beta 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Periostin (POSTN) Regulates Tumor Resistance to Antiangiogenic Therapy in Glioma Models
    Authors: Soon Young Park, Yuji Piao, Kang Jin Jeong, Jianwen Dong, John F. de Groot
    Molecular Cancer Therapeutics
  2. Dual-ligand modification of PEGylated liposomes shows better cell selectivity and efficient gene delivery
    Authors: Golam Kibria, Hiroto Hatakeyama, Noritaka Ohga, Kyoko Hida, Hideyoshi Harashima
    Journal of Controlled Release
  3. The Mechanically-induced Catalytic Amplification Reaction for Readout of Receptor-Mediated Cellular Forces
    Authors: Victor Pui‐Yan Ma, Yang Liu, Kevin Yehl, Kornelia Galior, Yun Zhang, Khalid Salaita
    Angewandte Chemie International Edition
  4. Potential of uPAR, alphavbeta6 Integrin, and Tissue Factor as Targets for Molecular Imaging of Oral Squamous Cell Carcinoma: Evaluation of Nine Targets in Primary Tumors and Metastases by Immunohistochemistry
    Authors: M Lawaetz, A Christense, K Juhl, K Karnov, G Lelkaitis, AM Kanstrup F, A Kjaer, C von Buchwa
    International Journal of Molecular Sciences, 2023-02-14;24(4):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
    Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
    Nature Immunology, 2022-12-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Direct targeting of oncogenic RAS mutants with a tumor-specific cytosol-penetrating antibody inhibits RAS mutant-driven tumor growth
    Authors: SM Shin, JS Kim, SW Park, SY Jun, HJ Kweon, DK Choi, D Lee, YB Cho, YS Kim
    Sci Adv, 2020-01-15;6(3):eaay2174.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. The post-abscission midbody is an intracellular signaling organelle that regulates cell proliferation
    Authors: E Peterman, P Gibieža, J Schafer, VA Skeberdis, A Kaupinis, M Valius, X Heiligenst, I Hurbain, G Raposo, R Prekeris
    Nat Commun, 2019-07-18;10(1):3181.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Acidic preconditioning of endothelial colony-forming cells (ECFC) promote vasculogenesis under proinflammatory and high glucose conditions in vitro and in vivo
    Authors: HA Mena, PR Zubiry, B Dizier, M Schattner, C Boisson-Vi, S Negrotto
    Stem Cell Res Ther, 2018-05-02;9(1):120.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. HLA Class II-Triggered Signaling Cascades Cause Endothelial Cell Proliferation and Migration: Relevance to Antibody-Mediated Transplant Rejection
    Authors: YP Jin, NM Valenzuela, X Zhang, E Rozengurt, EF Reed
    J. Immunol., 2018-02-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  10. Osteopontin-integrin engagement induces HIF-1?-TCF12-mediated endothelial-mesenchymal transition to exacerbate colorectal cancer
    Authors: CS Fan, WS Chen, LL Chen, CC Chen, YT Hsu, KV Chua, HD Wang, TS Huang
    Oncotarget, 2017-12-22;9(4):4998-5015.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. AlphaVBeta3 Integrin expression within uterine endometrium in unexplained infertility: a prospective cohort study
    Authors: A Elnaggar, AH Farag, ME Gaber, MA Hafeez, MS Ali, AM Atef
    BMC Womens Health, 2017-09-26;17(1):90.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. cRGD peptide installation on cisplatin-loaded nanomedicines enhances efficacy against locally advanced head and neck squamous cell carcinoma bearing cancer stem-like cells
    Authors: K Miyano, H Cabral, Y Miura, Y Matsumoto, Y Mochida, H Kinoh, K Iwata, O Nagano, H Saya, N Nishiyama, K Kataoka, T Yamasoba
    J Control Release, 2017-06-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Osteopontin Promotes Oncostatin M Production in Human Osteoblasts: Implication of Rheumatoid Arthritis Therapy.
    Authors: Su C, Chiang Y, Huang C, Hsu C, Fong Y, Tang C
    J Immunol, 2015-08-24;195(7):3355-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Arachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro.
    Authors: Kasonga A, Deepak V, Kruger M, Coetzee M
    PLoS ONE, 2015-04-13;10(4):e0125145.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  15. C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCzeta tumor-suppressive activities and regulating integrin affinity modulation.
    Authors: Zhang P, Fu C, Hu Y, Dong C, Song Y, Song E
    Sci Rep, 2015-03-20;5(0):9275.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells.
    Authors: Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N
    Cell Commun Signal, 2014-06-25;12(0):36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. The nexus between VEGF and NFkappaB orchestrates a hypoxia-independent neovasculogenesis.
    Authors: DeNiro M, Al-Mohanna F, Alsmadi O, Al-Mohanna F
    PLoS ONE, 2013-03-22;8(3):e59021.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. The role of RGD-tagged helical rosette nanotubes in the induction of inflammation and apoptosis in human lung adenocarcinoma cells through the P38 MAPK pathway.
    Authors: Suri SS, Rakotondradany F, Myles AJ, Fenniri H, Singh B
    Biomaterials, 2009-02-27;30(17):3084-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. The deubiquitinase USP10 regulates integrin beta1 and beta5 and fibrotic wound healing
    Authors: Stephanie R. Gillespie, Liana J. Tedesco, Lingyan Wang, Audrey M. Bernstein
    Journal of Cell Science

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