Home / Integrin beta 1/CD29 / Human Integrin  beta 1/CD29 Antibody

Human Integrin  beta 1/CD29 Antibody

Catalog #: MAB17781
25 Citations 4 Reviews Datasheet / COA / SDS
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MAB17781
MAB17781-SP
Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (25)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  beta 1/CD29 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 1/CD29.
Source
Monoclonal Mouse IgG1 Clone # P5D2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human skin keratinocytes
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunoprecipitation

Lin, Q. et al. (2004) Biochim. Biophys. Acta 1689:175.
Joneckis, C.C. et al. (1993) Blood 82:3548.

 
Blockade of Receptor-ligand Interaction
Mukhopadhyay, N.K. et al. (2004) Ann. Thorac. Surg. 78:450.
Blaschke, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:890.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Knockout Validated
Integrin beta 1/CD29 is specifically detected in A549 lung carcinoma parental cell line but is not detectable in Integrin beta 1/CD29 knockout A549 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin beta 1/CD29 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Flow Cytometry Detection of Integrin beta 1/CD29 antibody in A549 human lung carcinoma cell line antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in A549 human lung carcinoma cell line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry Integrin beta 1/CD29 antibody in human PBMCs by Immunocytochemistry (ICC). View Larger

Integrin beta 1/CD29 in human PBMCs. Integrin beta 1/CD29 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated Integrin beta 1/CD29 Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line. View Larger

Integrin beta 1/CD29 Specificity is Shown by Flow Cytometry in Knockout Cell Line. Integrin beta 1/CD29 knockout A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog# F0102B). No staining in the Integrin beta 1/CD29 knockout A549 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.

References
  1. Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.
  2. Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.
Entrez Gene IDs
3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)
Alternate Names
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

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Citations for Human Integrin  beta 1/CD29 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Sarcoma cells secrete hypoxia-modified collagen VI to weaken the lung endothelial barrier and promote metastasis
    Authors: Liu, Y;Murazzi, I;Fuller, AM;Pan, H;Irizarry-Negron, VM;DeVine, A;Katti, R;Skuli, N;Ciotti, GE;Pak, K;Pack, MA;Simon, MC;Weber, K;Cooper, K;Eisinger-Mathason, TSK;
    Cancer research
    Species: Human hepegivirus, Murine polyomavirus strain A3, rainbow trout
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
    Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
    Nature Immunology, 2022-12-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I
    Authors: G Liu, R Liu, Y Shan, C Sun
    The Journal of Biological Chemistry, 2021-08-28;0(0):101133.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Irisin supports integrin-mediated cell adhesion of lymphocytes
    Authors: PK Myint, A Ito, MG Appiah, G Obeng, S Darkwah, E Kawamoto, A Gaowa, EJ Park, M Shimaoka
    Biochemistry and Biophysics Reports, 2021-03-08;26(0):100977.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  5. The Lectin-Like Domain of Thrombomodulin Inhibits &beta1 Integrin-Dependent Binding of Human Breast Cancer-Derived Cell Lines to Fibronectin
    Authors: E Kawamoto, N Nago, T Okamoto, A Gaowa, A Masui-Ito, Y Akama, S Darkwah, MG Appiah, PK Myint, G Obeng, A Ito, S Caidengbat, R Esumi, T Yamaguchi, EJ Park, H Imai, M Shimaoka
    Biomedicines, 2021-02-07;9(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Neohesperidin Ameliorates Steroid-Induced Osteonecrosis of the Femoral Head by Inhibiting the Histone Modification of lncRNA HOTAIR
    Authors: S Yuan, C Zhang, Y Zhu, B Wang
    Drug Design, Development and Therapy, 2020-12-07;14(0):5419-5430.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells
    Authors: G Luthria, R Li, S Wang, M Prytyskach, RH Kohler, DA Lauffenbur, TJ Mitchison, R Weissleder, MA Miller
    Nat Commun, 2020-07-14;11(1):3521.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process
    Authors: SP Davies, GM Reynolds, AL Wilkinson, X Li, R Rose, M Leekha, YS Liu, R Gandhi, E Buckroyd, J Grove, NM Barnes, RC May, SG Hubscher, DH Adams, Y Huang, O Qureshi, Z Stamataki
    Cell Rep, 2019-11-05;29(6):1610-1620.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: IF
  9. Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells
    Authors: A Rajan, BD Persson, L Frängsmyr, A Olofsson, L Sandblad, J Heino, Y Takada, AP Mould, LM Schnapp, J Gall, N Arnberg
    Sci Rep, 2018-07-03;8(1):10019.
    Applications: Flow Cytometry
  10. Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin ?1 to Promote Embryo-tubal Attachment
    Authors: SF Ahmad, JK Brown, LL Campbell, M Koscielnia, C Oliver, N Wheelhouse, G Entrican, S McFee, GS Wills, MO McClure, PJ Horner, S Gaikoumelo, KF Lee, HOD Critchley, WC Duncan, AW Horne
    EBioMedicine, 2018-02-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Doxorubicin resistance in breast cancer cells is mediated by extracellular matrix proteins
    Authors: CJ Lovitt, TB Shelper, VM Avery
    BMC Cancer, 2018-01-06;18(1):41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Human adenovirus type 37 uses ?V?1 and ?3?1 integrins for infection of human corneal cells
    Authors: Rickard J Storm
    J. Virol, 2017-02-14;0(0):.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-Fr, Neutralization
  13. Fibronectin-guided migration of carcinoma collectives
    Authors: S Gopal, L Veracini, D Grall, C Butori, S Schaub, S Audebert, L Camoin, E Baudelet, A Radwanska, S Beghelli-d, SM Violette, PH Weinreb, S Rekima, M Ilie, A Sudaka, P Hofman, E Van Obberg
    Nat Commun, 2017-01-19;8(0):14105.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Identification of novel peptide motifs in the serpin maspin that affect vascular smooth muscle cell function
    Authors: R Bass
    Biochim. Biophys. Acta, 2016-11-23;1864(2):336-344.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes
    Sci Rep, 2016-09-27;6(0):34310.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity.
    Authors: Zhang, Qian, Dove, Christop, Hor, Jyh Lian, Murdock, Heardley, Strauss-Albee, Dara M, Garcia, Jordan A, Mandl, Judith N, Grodick, Rachael, Jing, Huie, Chandler-Brown, Devon B, Lenardo, Timothy, Crawford, Greg, Matthews, Helen F, Freeman, Alexandr, Cornall, Richard, Germain, Ronald N, Mueller, Scott N, Su, Helen C
    J Exp Med, 2014-11-24;211(13):2549-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  17. The alpha-subunit regulates stability of the metal ion at the ligand-associated metal ion-binding site in beta3 integrins.
    Authors: Rui X, Mehrbod M, Van Agthoven J, Anand S, Xiong J, Mofrad M, Arnaout M
    J Biol Chem, 2014-06-28;289(33):23256-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells.
    Authors: Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N
    Cell Commun Signal, 2014-06-25;12(0):36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Cultivation of corneal endothelial cells on a pericellular matrix prepared from human decidua-derived mesenchymal cells.
    Authors: Numata R, Okumura N, Nakahara M, Ueno M, Kinoshita S, Kanematsu D, Kanemura Y, Sasai Y, Koizumi N
    PLoS ONE, 2014-02-05;9(2):e88169.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants.
    Authors: Iwai L, Payne L, Luczynski M, Chang F, Xu H, Clinton R, Paul A, Esposito E, Gridley S, Leitinger B, Naegle K, Huang P
    Biochem J, 2013-09-15;454(3):501-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Degradation of internalized alphaVbeta5 integrin is controlled by uPAR bound uPA: effect on beta1 integrin activity and alpha-SMA stress fiber assembly.
    Authors: Wang L, Pedroja BS, Meyers EE, Garcia AL, Twining SS, Bernstein AM
    PLoS ONE, 2012-03-21;7(3):e33915.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Epstein-Barr virus infection of polarized epithelial cells via the basolateral surface by memory B cell-mediated transfer infection.
    Authors: Shannon-Lowe C, Rowe M
    PLoS Pathog., 2011-05-05;7(5):e1001338.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  23. The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
    Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
    Biol. Reprod., 2010-01-14;82(5):968-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009-07-30;51(4):734-49.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  25. The chemokine receptor CX3CR1 is involved in the neural tropism and malignant behavior of pancreatic ductal adenocarcinoma.
    Authors: Marchesi F, Piemonti L, Fedele G, Destro A, Roncalli M, Albarello L, Doglioni C, Anselmo A, Doni A, Bianchi P, Laghi L, Malesci A, Cervo L, Malosio M, Reni M, Zerbi A, Di Carlo V, Mantovani A, Allavena P
    Cancer Res., 2008-11-01;68(21):9060-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization

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Reviews for Human Integrin  beta 1/CD29 Antibody

Average Rating: 4.5 (Based on 4 Reviews)

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Human Integrin  beta 1/CD29 Antibody
By Anonymous on 03/20/2022
Application: WB Sample Tested: Tumor cell lysates Species: Human
Western Blot Human Integrin  beta 1/CD29 Antibody MAB17781
Human Integrin  beta 1/CD29 Antibody
By Anonymous on 04/02/2020
Application: WB Sample Tested: Mucociliary epithelium Species: Human
Western Blot Human Integrin  beta 1/CD29 Antibody MAB17781
Human Integrin  beta 1/CD29 Antibody
By Anonymous on 02/16/2020
Application: WB Sample Tested: Tumor cell lyastes Species: Human
Western Blot Human Integrin  beta 1/CD29 Antibody MAB17781
Human Integrin  beta 1/CD29 Antibody
By Anonymous on 04/24/2018
Application: WB Sample Tested: blood endothelial cells Species: Human

Worked in both reducing and non-reducing conditions
Band seen around 130kDa
Would use again on a new blot, not a stripped blot as got very similar band to that with alpha 5

Western Blot Human Integrin  beta 1/CD29 Antibody MAB17781