Human Integrin beta 1/CD29 Biotinylated Antibody

Catalog #: BAF1778
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF1778

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry.

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Citations (4)

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Human Integrin beta 1/CD29 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin beta 1 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) Integrin beta 1 is observed and less than 1% cross-reactivity recombinant human (rh) Integrin beta 2, rhIntegrin beta 3, rmIntegrin beta 6, and rmIntegrin beta 7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1 isoform 1A
Gln21-Asp728
Accession # P05556

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Integrin beta 1/CD29

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human Integrin beta 1/CD29 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1778, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

Integrin beta 1, also called CD29 and VLA-beta chain, associates with at least ten different integrin alpha subunits to form various VLA complexes. The beta 1 subunit has a broad tissue distribution except erythrocytes.

Entrez Gene IDs

3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)

Alternate Names

CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Integrin beta 1/CD29 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Interaction of Pregnancy-Specific Glycoprotein 1 With Integrin alpha5beta1 Is a Modulator of Extravillous Trophoblast Functions
Authors: S Rattila, CEE Dunk, M Im, O Grichenko, Y Zhou, M Yanez-Mo, SM Blois, KM Yamada, O Erez, N Gomez-Lope, SJ Lye, B Hinz, R Romero, M Cohen, G Dveksler
Cells, 2019-10-31;8(11):.
Species: Human
Sample Types:
Applications: ELISA Detection
Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells
Authors: D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio
J. Clin. Invest., 2019-05-14;129(7):2745-2759.
Species: Human
Sample Types: Protein
Applications: Bioassay
Monoclonal antibodies to human laminin alpha4 chain globular domain inhibit tumor cell adhesion and migration on laminins 411 and 421, and binding of alpha6beta1 integrin and MCAM to alpha4-laminins.
Authors: Ishikawa T, Wondimu Z, Oikawa Y, Ingerpuu S, Virtanen I, Patarroyo M
Matrix Biol, 2014-03-27;36(0):5-14.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development (Detection)
CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients.
Authors: Martinez-Vinambres E, Garcia-Trujillo JA, Rodriguez-Martin E, Villar LM, Coll J, Roldan E
Leukemia, 2011-12-02;26(5):1098-105.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot