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Human Integrin  beta 3/CD61 Antibody

Catalog #: AF2266
3 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2266
AF2266-SP
Detection of Human Integrin beta 3/CD61 by Western Blot.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  beta 3/CD61 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 3/CD61 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Integrin beta 2 and recombinant mouse (rm) Integrin beta 6 is observed and less than 1% cross-reactivity with rhIntegrin beta 1 and rmIntegrin  beta 4 is observed.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
U‑87 MG human glioblastoma/astrocytoma cell line
Flow Cytometry
0.25 µg/106 cells
Human peripheral blood mononuclear cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Integrin beta 3/CD61 antibody by Western Blot. View Larger

Detection of Human Integrin beta 3/CD61 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Integrin beta 3/ CD61 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2266) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Integrin beta 3/CD61 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human Integrin  beta 3/CD61 by Simple WesternTM. Simple Western lane view shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin  beta 3/CD61 at approximately 127 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Integrin  beta 3/CD61 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2266). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Integrin beta 3/CD61

Integrin beta 3, also called CD61, associates with the Integrin alpha IIb or alpha V subunits to form heterodimeric receptors for collagens, fibronectin, vitronectin, fibrinogen, Von willebrand factor or thrombospondin. alpha IIb/ beta 3, also known as GPIIb IIIa (Glycoprotein IIb-IIIa), is expressed on platelets. alpha V/ beta 3, also known as vitronectin receptor, is expressed on endothelial cells, some B cells, platelets and monocytes.

Entrez Gene IDs
3690 (Human); 16416 (Mouse); 29302 (Rat); 102117791 (Cynomolgus Monkey)
Alternate Names
CD61 antigen; CD61; GP3A; GP3Aplatelet glycoprotein IIIa; GPIIIA; GPIIIaintegrin beta-3; INGRB3; Integrin beta 3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); ITG B3; ITGB3; Platelet membrane glycoprotein IIIa

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Citations for Human Integrin  beta 3/CD61 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species
    Authors: Tabebordbar M, Lagerborg KA, Stanton A et al.
    Cell
  2. Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood
    Authors: WM Dickerson, R Yu, HU Westergren, J Paraskos, P Schatz, A Tigerstrom, A Ekman, J Sánchez, J Cheng, L Li, EY Chan
    PLoS ONE, 2021-08-26;16(8):e0256423.
    Species: Human
    Sample Types: Plasma
    Applications: Interferent Testing
  3. Platelet-derived extracellular vesicles are increased in sera of Alzheimer's disease patients, as revealed by Tim4-based assays
    Authors: H Odaka, K Hiemori, A Shimoda, K Akiyoshi, H Tateno
    FEBS Open Bio, 2021-02-03;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human Integrin  beta 3/CD61 Antibody
By Anonymous on 09/14/2019
Application: WB Sample Tested: Cell Lysates Species: Human
Western Blot Human Integrin  beta 3/CD61 Antibody AF2266