Human Integrin beta 4/CD104 Antibody Summary
Asn28-Ser710
Accession # P16144
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Integrin beta 4/CD104 in A431 Human Cell Line. Integrin beta 4/CD104 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4060) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 4/CD104
Integrin beta 4 (also GP150 and CD104) is a 150‑200 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human Integrin beta 4 is a type I transmembrane glycoprotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28‑710) and a large 1089 aa cytoplasmic region. Two ECD alternative splice forms may exist. One shows an 84 aa substitution for the 84 aa between aa 621‑704. A second shows a 144 aa substitution for the first 704 amino acids. Human beta 4 ECD shares 88% aa sequence identity with mouse beta 4 ECD.
Product Datasheets
Citations for Human Integrin beta 4/CD104 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Human Basal and Suprabasal Keratinocytes Are Both Able to Generate and Maintain Dermo-Epidermal Skin Substitutes in Long-Term In Vivo Experiments
Authors: L Pontiggia, AK Ahuja, HK Yosef, D Rütsche, E Reichmann, U Moehrlen, T Biedermann
Cells, 2022-07-09;11(14):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression
Authors: DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger
The Journal of Biological Chemistry, 2022-05-13;0(0):102029.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells
Authors: CR Keller, Y Hu, KF Ruud, AE VanDeen, SR Martinez, BT Kahn, Z Zhang, RK Chen, W Li
Cancers, 2021-11-22;13(22):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
Authors: R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu
PLoS ONE, 2019-05-02;14(5):e0215990.
Species: Human
Sample Types: Plasma
Applications: ELISA Detection -
Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells
Authors: A Rajan, BD Persson, L Frängsmyr, A Olofsson, L Sandblad, J Heino, Y Takada, AP Mould, LM Schnapp, J Gall, N Arnberg
Sci Rep, 2018-07-03;8(1):10019.
Applications: Flow Cytometry -
Granzyme B is elevated in autoimmune blistering diseases and cleaves key anchoring proteins of the dermal-epidermal junction
Authors: V Russo, T Klein, DJ Lim, N Solis, Y Machado, S Hiroyasu, L Nabai, Y Shen, MR Zeglinski, H Zhao, CP Oram, PA Lennox, N Van Laeken, NJ Carr, RI Crawford, CW Franzke, CM Overall, DJ Granville
Sci Rep, 2018-06-26;8(1):9690.
Applications: Western Blot -
Human adenovirus type 37 uses ?V?1 and ?3?1 integrins for infection of human corneal cells
Authors: Rickard J Storm
J. Virol, 2017-02-14;0(0):.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC-Fr, Neutralization -
Integrin alpha6beta4 identifies human distal lung epithelial progenitor cells with potential as a cell-based therapy for cystic fibrosis lung disease.
Authors: Li X, Rossen N, Sinn P, Hornick A, Steines B, Karp P, Ernst S, Adam R, Moninger T, Levasseur D, Zabner J
PLoS ONE, 2013-12-12;8(12):e83624.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Integrin alpha5beta1 facilitates cancer cell invasion through enhanced contractile forces.
Authors: Mierke CT, Frey B, Fellner M, Herrmann M, Fabry B
J. Cell. Sci., 2011-01-11;124(0):369-83.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
