Human Intelectin-2 Antibody

Catalog #: MAB8004 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8004
MAB8004-SP

Detection of Human Intelectin‑2 by Western Blot.

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Human Intelectin-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Intelectin-2 in ELISA. In Direct ELISA, no cross-reactivity with recombinant human (rh) Intelectin-1, recombinant mouse (rm) Intelectin-1, or rmIntelectin-2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 874029

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Intelectin-2
Met1-Tyr324
Accession # Q8WWU7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Intelectin‑2 by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Intelectin-2 Monoclonal Antibody (Catalog # MAB8004) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Intelectin-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Intelectin‑2 in Human Colon. Intelectin-2 was detected in formalin fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human Intelectin-2 Monoclonal Antibody (Catalog # MAB8004) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Intelectin-2

Intelectin-2 (ITLN-2; also endothelial lectin 2 and HL-2) is a 37-41 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by intestinal Paneth cells, and appears to participate in fungal microbe recognition. The human ITLN-2 precursor is 325 amino acids (aa) in length (SwissProt #:Q8WWU7). It contains a 26 aa signal sequence and a 299 aa mature region. Although mouse ITLN-2 is reportedly GPI-linked, and mouse and human ITLN-2 show considerable C-terminal aa identity, it is not known if human ITNL-2 is also GPI-linked. There is one fibrinogen-related domain in the mature molecule (aa 44-267). One potential splice variant is reported that shows a premature truncation after Gly264. Over aa 29-324, human ITLN-2 shares 79% aa sequence identity with mouse ITLN-2/ITLN-1b, and 85% aa sequence identity with human ITLN-1.

Entrez Gene IDs

142683 (Human); 493583 (Mouse)

Alternate Names

HL-2; intelectin 2; Intelectin2; Intelectin-2; Intelectin-b; Itln1b; ITLN2; Itlnb; UNQ2789/PRO7179