Human IRF4 Antibody

Name: Human IRF4 Antibody
Brand: R&D Systems
SKU: MAB55251
Rating: 5 (1 reviews)

Catalog #: MAB55251 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB55251-100
MAB55251-SP

Detection of Human IRF4 by Western Blot.

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All IRF4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IRF4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IRF4 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 503210

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IRF4
Glu130-Glu451
Accession # Q15306

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Ramos human Burkitt's lymphoma cell line and HDLM‑2 human Hodgkin's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IRF4 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, HDLM‑2 human Hodgkin’s lymphoma cell line, U937 human histiocytic lymphoma cell line (negative control), and HUVEC human umbilical vein endothelial cells (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IRF4 Monoclonal Antibody (Catalog # MAB55251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for IRF4 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF4

Interferon Regulatory Factor 4 (IRF4), also known as MUM1 and LSIRF, is a 51 kDa lymphocyte-restricted transcription factor. It is required for immunoglobulin class switching and terminal differentiation of B cells. IRF4 is overexpressed in multiple myeloma and cooperates with Myc in an autoregulatory loop. In T cells, IRF4 is required for the production of IL-4. IRF4 contains an N-terminal DNA binding domain that is homologous to that in other IRF proteins. Within the C-terminal domain (aa 130‑451), human IRF4 shares 90% aa sequence identity with mouse and rat IRF4. Alternate splicing may generate isoforms with N-terminal, C-terminal, or internal deletions.

Long Name

Interferon Regulatory Factor 4

Entrez Gene IDs

3662 (Human); 16364 (Mouse); 291100 (Rat)

Alternate Names

interferon regulatory factor 4; IRF4; IRF-4; LSIRF; LSIRFMUM1; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM1; NF-EM5