Human Kallikrein 11 Antibody

Catalog #: MAB15951 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB15951
MAB15951-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 11 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 11 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 371604

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 11
Glu51-Asn282
Accession # Q9UBX7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize Recombinant Human Kallikrein 11 (1 µg/mL, Catalog # 1595-SE) cleavage of the fluorogenic peptide substrate VLK-SBzl (0.1 mM). The Neutralization Dose (ND₅₀) is typically 2.4 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 11

As a member of human tissue kallikrein family, Kallikrein 11, also known as hippostasin, trypsin-like serine protease and PRSS20, is encoded by the KLK11 gene (1). Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences,respectively (2-5). Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue 1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas (6-8). Recombinant human (rh) KLK11, after being activated by thermolysin, is active against a thioester substrate. This activity can be inhibited by AEBSF (R&D Systems, Catalog # EI001), dichloroisocoumarin, and aprotinin. rhKLK11 produced by R&D Systems corresponds to isoform 1.

References

Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
Yoshida, S. et al. (1998) Biochim. Biophys. Acta 1399:225.
Yousef, G.M. et al. (2000) Genomics 63:88.
Mitsui, S. et al. (2000) Biochem. Biophys. Res. Commun. 272:205.
Gan, L. et al. (2000) Gene 257:119.
Diamandis, E.P. et al. (2002) Cancer Res. 62:295.
Nakamura, T. et al. (2003) Urology 61:1042.
Borgono, C.A. et al. (2003) Int. J. Cancer 106:605.

Entrez Gene IDs

11012 (Human)

Alternate Names

EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; Hippostasin; hK11; Kallikrein 11; kallikrein-related peptidase 11; KLK11; MGC33060; PRSS20kallikrein-11; Serine protease 20; TLSPhippostasin; Trypsin-like protease