Human Kallikrein 11 Biotinylated Antibody

Catalog #: BAF1595 Datasheet / COA / SDS

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BAF1595

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Human Kallikrein 11 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 11 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human (rh) Kallikrein 3 is observed and less than 1% cross-reactivity with rhKallikrein 5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 11
Ile54-Asn282
Accession # Q9UBX7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Kallikrein 11 (Catalog # 1595-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 11

As a member of human tissue kallikrein family, Kallikrein 11, also known as hippostasin, trypsin-like serine protease and PRSS20, is encoded by the KLK11 gene (1). Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences,respectively (2-5). Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue 1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas (6-8). Recombinant human (rh) KLK11, after being activated by thermolysin, is active against a thioester substrate. This activity can be inhibited by AEBSF (R&D Systems, Catalog # EI001), dichloroisocoumarin, and aprotinin. rhKLK11 produced by R&D Systems corresponds to isoform 1.

References

Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
Yoshida, S. et al. (1998) Biochim. Biophys. Acta 1399:225.
Yousef, G.M. et al. (2000) Genomics 63:88.
Mitsui, S. et al. (2000) Biochem. Biophys. Res. Commun. 272:205.
Gan, L. et al. (2000) Gene 257:119.
Diamandis, E.P. et al. (2002) Cancer Res. 62:295.
Nakamura, T. et al. (2003) Urology 61:1042.
Borgono, C.A. et al. (2003) Int. J. Cancer 106:605.

Entrez Gene IDs

11012 (Human)

Alternate Names

EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; Hippostasin; hK11; Kallikrein 11; kallikrein-related peptidase 11; KLK11; MGC33060; PRSS20kallikrein-11; Serine protease 20; TLSPhippostasin; Trypsin-like protease