Human Kallikrein 7 Antibody

Catalog #: MAB26242 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB26242-100
MAB26242-SP

Human Kallikrein 7 ELISA Standard Curve.

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All Kallikrein 7 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 7 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 333925

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 7
Glu23-His252
Accession # P49862

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody whenpaired with human anti Kallikrein 7 Monoclonal Antibody (Catalog # MAB26241).Thisproduct is intended for assay development on various assay platforms requiringantibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Kallikrein 7 ELISA Standard Curve. Recombinant Human Kallikrein 7 protein was serially diluted 2-fold and captured by Mouse Anti-Human Kallikrein 7 Monoclonal Antibody (MAB26241) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Kallikrein 7 Monoclonal Antibody (Catalog # MAB26242) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 7

Human tissue Kallikrein 7 (hK7), also known as stratum corneum chymotryptic enzyme (SCCE), is a member of the human tissue kallikrein family. Full-length Kallikrein 7 consists of 253 amino acids (aa), with a signal peptide (aa 1-22), short pro peptide (aa 23-29) and mature chain (aa 30-252) (1). Predominantly expressed in the skin, a major physiological function of Kallikrein 7 is to regulate the desquamation process through proteolysis of the intercellular adhesive structures between corneocytes (2). Thus, it is related to some inflammatory skin diseases, such as psoriasis and chronic itchy dermatitis (3, 4). Studies have shown that one potential physiological activator for Kallikrein 7 is Kallikrein 5, another member of the human tissue Kallikrein family. Along with Kallikrein 14, these three Kallikreins form a proteolytic cascade in the stratum corneum (5).

References

Hansson, L. et al. (1994) J. Bio. Chem. 269:19420.
Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
Ekholm, E. and T. Egelrud (1999) Arch. Dermatol. Res. 291:195.
Hansson, L. et al. (2002) J. Invest. Dermatol. 118:444.
Brattsand, M. et al. (2004) J. Invest. Dermatol. 124:198.

Entrez Gene IDs

5650 (Human); 23993 (Mouse)

Alternate Names

EC 3.4.21; EC 3.4.21.117; hK7; hSCCE; kallikrein 7 (chymotryptic, stratum corneum); Kallikrein 7; kallikrein-related peptidase 7; KLK7; protease, serine, 6; PRSS6; SCCEkallikrein-7; Serine protease 6; signal protein; Stratum corneum chymotryptic enzyme