Human Kininogen Light Chain Antibody

Catalog #: MAB15691 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB15691
MAB15691-SP

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Human Kininogen Light Chain Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kininogen Light Chain in direct ELISAs and Western blots. In Western blots, it reacts with the full length (amino acids 19‑644) and two forms of the light chain starting at amino acids 390 and 438, respectively, and does not react with the E. coli-expressed D5/Kininostatin. In Western blots, no cross-reactivity with recombinant human (rh) Cystatins A, B, C, D, E/M, F, S, SA, SN, rhFetuins A, B, rhHPRG, or recombinant mouse Kininogen is observed.

Source

Monoclonal Mouse IgG_2B Clone # 236006

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kininogen
Gln19-Ser644 (Ile581Thr) (predicted)
Accession # P01042.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Kininogen High Molecular Weight (HKa) (Catalog # 1569-PI)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Kininogen High Molecular Weight (HKa) (Catalog # 1569-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kininogen

Kininogen, also known as alpha 2-thiol proteinase inhibitor, is a multi-function protein. There are two alternatively spliced forms, designated as the high molecular weight (HMW) and low MW (LMW) forms (1). The HMW form is synthesized as a 644 amino acid (aa) precursor with a signal peptide (aa 1‑18). The mature chain (aa 19‑644) is further processed into the heavy (aa 19‑380) and the light (aa 390‑644) chains. The active peptide bradykinin (aa 381‑389) is released, which has a variety of functions including muscle contraction, hypotension and inflammation. The heavy chain consists of three cystatin-like domains, which are responsible for inhibiting cysteine proteases. The light chain consists of a His-rich domain, which is associated with the clotting activity. In comparison to the HMW form, the LMW Kininogen (427 aa) has the same sequence in its heavy chain and bradykinin, but a different sequence in its light chain (aa 402‑427). The LMW form is not involved in blood clotting.

References