Home / KIR2DL1/KIR2DS5 / Human KIR2DL1/KIR2DS5 APC-conjugated Antibody

Human KIR2DL1/KIR2DS5 APC-conjugated Antibody

Catalog #: FAB1844A
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1844A-025
FAB1844A-100
Detection of KIR2DL1/KIR2DS5 in Human PBMCs by Flow Cytometry.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KIR2DL1/KIR2DS5 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR2DL1/KIR2DS5 on transfected cells. It does not stain cells transfected with KIR2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
Source
Monoclonal Mouse IgG1 Clone # 143211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DL1/KIR2DS5
Accession # P43626
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of KIR2DL1/KIR2DS5 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of KIR2DL1/KIR2DS5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR2DL1/KIR2DS5 APC-conjugated Monoclonal Antibody (Catalog # FAB1844A) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DL1/KIR2DS5

KIR2DL1 is one of several immunoglobulin-like receptors expressed on NK cells that bind MHC class I molecules and transmit inhibitory signals. KIR2DL1 is specific for HLA-C alleles with Asn77 and Lys80.

Alternate Names
CD158a; KIR2DL1/KIR2DS5

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Citations for Human KIR2DL1/KIR2DS5 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. NK Cell Responses to Human Tick-Borne Encephalitis Virus Infection
    J Immunol, 2016-08-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells.
    Authors: Marquardt N, Beziat V, Nystrom S, Hengst J, Ivarsson M, Kekalainen E, Johansson H, Mjosberg J, Westgren M, Lankisch T, Wedemeyer H, Ellis E, Ljunggren H, Michaelsson J, Bjorkstrom N
    J Immunol, 2015-02-11;194(6):2467-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Tracking in vivo dynamics of NK cells transferred in patients undergoing stem cell transplantation.
    Authors: Killig M, Friedrichs B, Meisig J, Gentilini C, Bluthgen N, Loddenkemper C, Labopin M, Basara N, Pfrepper C, Niederwieser D, Uharek L, Romagnani C
    Eur J Immunol, 2014-06-30;44(9):2822-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection.
    Authors: Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M
    Eur J Immunol, 2012-12-12;43(2):480-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. CMV drives clonal expansion of NKG2C+ NK cells expressing self-specific KIRs in chronic hepatitis patients.
    Authors: Beziat V, Dalgard O, Asselah T, Halfon P, Bedossa P, Boudifa A, Hervier B, Theodorou I, Martinot M, Debre P, Bjorkstrom NK, Malmberg KJ, Marcellin P, Vieillard V
    Eur. J. Immunol., 2011-12-16;42(2):447-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs.
    Authors: Beziat V, Descours B, Parizot C, Debre P, Vieillard V
    PLoS ONE, 2010-08-06;5(8):e11966.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Autologous antitumor activity by NK cells expanded from myeloma patients using GMP-compliant components.
    Authors: Alici E, Sutlu T, Bjorkstrand B, Gilljam M, Stellan B, Nahi H, Quezada HC, Gahrton G, Ljunggren HG, Dilber MS
    Blood, 2008-01-11;111(6):3155-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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