Human KIR2DS1/CD158h PE-conjugated Antibody

Catalog #: FAB8887P
1 Citation Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
FAB8887P-025
FAB8887P-100

Detection of KIR2DS1/CD158h in Human PBMCs by Flow Cytometry.

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Human KIR2DS1/CD158h PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human KIR2DS1/CD158h in flow cytometry. Clone 1127B recognizes KIR2DS1 and some alleles of KIR2DL1, both members of the killer cell immunoglobulin-like receptor (KIR) family. Because Clone 1127B displays partial cross-reactivity with KIR2DL1, co-staining with Mouse Anti-Human KIR2DL1 Clone 143211 (Catalog # FAB1844A) is recommended.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1127B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human KIR2DS1/CD158h
Met1-His245
Accession # Q14954

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of KIR2DS1/CD158h in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Rabbit Anti-Human KIR2DS1/CD158h PE-conjugated Monoclonal Antibody (Catalog # FAB8887P) and either (A) Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) or (B) Mouse Anti-Human KIR2DL1/KIR2DS5 APC-conjugated Monoclonal Antibody (Catalog # FAB1844A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DS1/CD158h

The KIRs comprise a family of 14 polymorphic and homologous activating and inhibitory receptors expressed primarily on CD56^dim NK cells. KIR2DS1 is an activating receptor with high homology to the inhibitory receptor KIR2DL1. Both KIR2DL1 and KIR2DS1 bind to HLA-C alleles containing the C2 epitope. KIR2DS1 and KIR2DL1 expression regulates NK cell licensing and activation in a number of immune contexts. Over amino acids (aa) 1-245, human KIR2DS1 shares 97% aa sequence identity with human KIR2DL1.

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 1

Entrez Gene IDs

3806 (Human)

Alternate Names

killer cell immunoglobulin-like receptor 2DS1; CD158a; CD158h; EB6ActI; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 1; KIR2DS1; p50.1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human KIR2DS1/CD158h PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

High-Resolution Genetic and Phenotypic Analysis of KIR2DL1 Alleles and Their Association with Pre-Eclampsia
Authors: O Huhn, O Chazara, MA Ivarsson, C Retière, TC Venkatesan, PJ Norman, HG Hilton, J Jayaraman, JA Traherne, J Trowsdale, M Ito, C Kling, P Parham, H Ghadially, A Moffett, AM Sharkey, F Colucci
J. Immunol., 2018-09-24;0(0):.
Species: Human
Sample Types: Whole Cells