Human KLF2 Antibody

Catalog #: MAB5466
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5466
MAB5466-SP

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All KLF2 Antibodies

Product Details

Citations (2)

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Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KLF2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human KLF2 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) KLF17 and no cross-reactivity with rhKLF1, 4, 5, 6, 10, 12, 17, recombinant mouse KLF4 or 15 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 665333

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human KLF2
Pro71-Pro168 (predicted)
Accession # Q9Y5W3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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KLF2 in BG01V Human Cells. KLF2 was detected in immersion fixed BG01V human embryonic stem cells using Human KLF2 Monoclonal Antibody (Catalog # MAB5466) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry

Detection of KLF2 antibody in BG01V Human Cells antibody by Flow Cytometry.

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Detection of KLF2 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human KLF2 Monoclonal Antibody (Catalog # MAB5466, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLF2

KLF2 (Kruppel-like factor 2; also LKLF) is a lung-associated, 37 kDa member of the Kruppel C2H2-type zinc-finger protein family. KLF2 is found in airway epithelium, endothelium, monocytes, T and B cells. It is a transcription factor that regulates multiple genes, many of which are involved in cell migration. Human KLF2 is 355 amino acids (aa) in length. It contains an activation domain (aa 1-110), an inhibitory domain (aa 111-267), and three C2H2-type zinc-finger regions (aa 272-354). There is one potential splice form that shows a premature truncation after Asp224. Over aa 71-168, human KLF2 is 82% aa identical to mouse KLF2.

Long Name

Kruppel-Like Factor 2

Entrez Gene IDs

10365 (Human); 16598 (Mouse); 306330 (Rat)

Alternate Names

KLF2; Krueppel-like factor 2; Kruppel-like factor 2 (lung); Kruppel-like factor LKLF; LKLF; LKLFLung krueppel-like factor; lung Kruppel-like zinc finger transcription factor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human KLF2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Memantine protects against ischemia/reperfusion-induced brain endothelial permeability
Authors: Y Liu, Y Huang, Y Xu, P Qu, M Wang
IUBMB Life, 2018-03-15;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Cellular origin and pathophysiology of chronic lymphocytic leukemia.
Authors: Seifert M, Sellmann L, Bloehdorn J, Wein F, Stilgenbauer S, Durig J, Kuppers R
J Exp Med, 2012-10-22;209(12):2183-98.
Species: Human
Sample Types: Whole Cells
Applications: ICC