Human KLRG1 Antibody

Catalog #: MAB70293
1 Citation Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB70293-100
MAB70293-SP

Detection of KLRG1 in Human Peripheral Blood by Flow Cytometry.

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All KLRG1 Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KLRG1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human KLRG1 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2388C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human KLRG1
Leu60-Phe195
Accession # Q96E93

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

3-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of KLRG1 in Human Peripheral Blood by Flow Cytometry. Human peripheral blood was stained with (A) Rabbit anti-Human KLRG1 Monoclonal Antibody (Catalog # MAB70293) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) and CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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KLRG1 in Human Tonsil. KLRG1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human KLRG1 Monoclonal Antibody (Catalog # MAB70293) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLRG1

KLRG1 (killer cell lectin-like receptor G1), also called MAFA (mast cell function associated), is a 30-38 kDa type II transmembrane inhibitory glycoprotein of the C‑type lectin family, designated CLEC15A. KLRG1 cDNA encodes 195 amino acids (aa) including an intracellular ITIM motif and a 136 aa extracellular domain (ECD) with a single C‑type lectin domain. The human KLRG1 ECD shares 57% and 54% aa identity with mouse and rat KLRG1, respectively. A 189 aa isoform diverges at aa 186. KLRG1 binds E-, N- and R-cadherins and functions as an MHC‑independent means of identifying non-self pathogens and epithelial tumor cells with low E‑cadherin expression. It is expressed as a monomer or disulfide‑linked homodimer on NK and T cell subsets such as tumor-infiltrating lymphocytes.

Long Name

Killer Cell Lectin-like Receptor Subfamily G Member 1

Entrez Gene IDs

10219 (Human); 50928 (Mouse); 58975 (Rat)

Alternate Names

2F1; CLEC15A; CLEC15AMAFA-LIKE; C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; ITIM-containing receptor MAFA-L; killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; KLRG1; MAFA; MAFAL; MAFA-L; MAFA-like receptor; MAFAMAFA-2F1; mast cell function-associated antigen (ITIM-containing); Mast cell function-associated antigen; MGC13600

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human KLRG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Neurodegeneration by alpha-synuclein-specific T cells in AAV-A53T-alpha-synuclein Parkinson's disease mice
Authors: AA Karikari, RL McFleder, E Ribechini, R Blum, V Bruttel, S Knorr, M Gehmeyr, J Volkmann, JM Brotchie, F Ahsan, B Haack, CM Monoranu, U Keber, R Yeghiazary, A Pagenstech, T Heckel, T Bischler, J Wischhusen, JB Koprich, MB Lutz, CW Ip
Brain, Behavior, and Immunity, 2022-01-12;101(0):194-210.
Species: Human
Sample Types: Whole Tissue
Applications: IHC