Human KOR Antibody

Catalog #: MAB3895
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3895
MAB3895-SP

KOR in HEK293 Human Cell line Transfected with KOR.

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All KOR Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KOR Antibody Summary

Species Reactivity

Human

Specificity

Detects human KOR. Stains human KOR transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG₁ Clone # 387301

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human KOR
Met1-Val380
Accession # P41145

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human KOR transfected HEK293 cells

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

Immersion fixed HEK293 human embryonic kidney cell line transfected with KOR

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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KOR in HEK293 Human Cell line Transfected with KOR. KOR was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with KOR (positive staining) and HEK293 human embryonic kidney cell line (wild type, negative staining) using Mouse Anti-Human KOR Monoclonal Antibody (Catalog # MAB3895) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KOR

KOR is a 45 kDa 7TM opioid receptor that is primarily expressed in the central nervous system and peripheral visceral pain sensory nerves. Following ligation by dynorphin peptides, KOR signaling induces analgesia, dysphoria, diuresis, and increased feeding desire. KOR also exerts neuroprotective and anti-inflammatory effects. Human KOR shares 94% amino acid sequence identity with mouse and rat KOR.

Long Name

Kappa Opioid Receptor

Entrez Gene IDs

4986 (Human); 18387 (Mouse); 29335 (Rat)

Alternate Names

kappa opioid receptor; kappa-type opioid receptor; KOR; K-OR-1; KOR-1; Opiate receptor, kappa-1; opioid receptor, kappa 1; OPRK; OPRK1; Oprk2; R21

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human KOR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

The atypical chemokine receptor ACKR3/CXCR7 is a broad-spectrum scavenger for opioid peptides
Authors: M Meyrath, M Szpakowska, J Zeiner, L Massotte, MP Merz, T Benkel, K Simon, J Ohnmacht, JD Turner, R Krüger, V Seutin, M Ollert, E Kostenis, A Chevigné
Nat Commun, 2020-06-19;11(1):3033.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry