Human Laminin alpha 1 N-Terminus Domain VI Antibody

Catalog #: AF4187 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4187
AF4187-SP

Detection of Laminin alpha 1 in U2OS Human Cell Line by Flow Cytometry.

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Human Laminin alpha 1 N-Terminus Domain VI Antibody Summary

Species Reactivity

Human

Specificity

Detects human Laminin alpha 1 N-Terminus Domain VI in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse LAMA4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Laminin alpha 1 N-Terminus Domain VI
Leu22-Met269
Accession # P25391

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Laminin alpha 1 N-Terminus Domain VI

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of mouse embryo (E10)

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Laminin alpha 1 antibody in U2OS Human Cell Line antibody by Flow Cytometry.

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Detection of Laminin alpha 1 in U2OS Human Cell Line by Flow Cytometry. U2OS human osteosarcoma cell line was stained with Goat Anti-Human Laminin alpha 1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF4187, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by APC-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 1

Laminin subunit alpha 1 (LAMA1) is a secreted 400 kDa extracellular matrix glycoprotein that contributes to the formation of basement membrane Laminin isoforms 1 and 3. It is one of three subunits ( alpha, beta, and gamma ) that interact via their coiled-coil domains to form the approximately 800 kDa cruciform, disulfide-linked, Laminin heterotrimer. The 3058 amino acid (aa) residue mature human alpha 1 chain contains an N-terminal Laminin VI domain (aa 18‑269), followed by domains V through III containing 17 EGF-like repeats, the coiled-coil domains II and I, and five globular, Laminin G-like domains. Over aa 22‑269, human Laminin alpha 1 shares 95% and 91% aa sequence identity with canine and mouse alpha 1 chain, respectively.

Entrez Gene IDs

284217 (Human); 16772 (Mouse); 316758 (Rat)

Alternate Names

LAMA; LAMA1 laminin, alpha 1 ; LAMA1; Laminin alpha 1; S-LAM-alpha