Human LAMP Antibody

Catalog #: MAB873 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB873
MAB873-SP

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Human LAMP Antibody Summary

Species Reactivity

Human

Specificity

Detects human LAMP in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 110728

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAMP
Val29-Asn315 (predicted)
Accession # AAC50569

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from ascites

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human LAMP Fc Chimera (Catalog # 873-LP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAMP

LAMP (limbic system-associated membrane protein) is a member of the IgLON (immunoglobulin LAMP, OBCAM and neurotrimin) subfamily within the Ig superfamily. All IgLON family members are glycosylphosphatidylinositol (GPI)-anchored neural cell adhesion molecules that are involved in cell-cell recognition and may have a role in mediating selective neuronal growth and axon targeting. LAMP cDNA encodes a 338 amino acid (aa) residues precursor protein containing a 28 aa N-terminal signal peptide, a 23 aa C-terminal propeptide and a 287 aa mature chain with 3 Ig-like C2-type domains and a GPI-anchor attachment site. In the developing brain, LAMP exhibits a specific pattern of expression in the cortical and subcortical limbic areas, which are important in cognition, emotion, memory, and learning. LAMP is also expressed in single layers of the superior colliculus, spinal chord and cerebellum. LAMP promotes adhesion and growth of limbic axons primarily via homophilic interaction and in part by modulating calcium influx through L-type calcium channels in limbic neurons. Heterophilic interactions between LAMP and neurotrimin have also been demonstrated. LAMP has been shown to inhibit the outgrowth of neurotrimin-expressing dorsal root ganglion neurons in a heterophilic manner. Antibody perturbation studies showed that LAMP is necessary for normal circuit formation in the limbic system, including the septo-hippocampal connection and hippocampal mossy fibers. LAMP acts as an attractive guidance signal for the limbic thalamic axons and can induce branch formation, but also acts as a repulsive axon guidance signal for nonlimbic thalamic axons (1‑5).

References