Human LCoR Antibody

Catalog #: MAB7534 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7534
MAB7534-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LCoR Antibody Summary

Species Reactivity

Human

Specificity

Detects human LCoR in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 731229

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human LCoR
Asn110-Gln338
Accession # Q96JN0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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LCoR in MCF‑7 Human Cell Line. LCoR was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human LCoR Monoclonal Antibody (Catalog # MAB7534) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LCoR

LCoR (ligand-dependent corepressor), also known as MLR2 (Mblk1-related protein 2), is a 47-50 kDa nuclear phosphoprotein that is a corepressor of ligand-dependent transcriptional activation by nuclear receptors such as estrogen and progesterone receptors. It is widely expressed throughout fetal development and in the adult. The 433 amino acid (aa) LCoR contains two N-terminal PxDLS nuclear receptor interaction motifs, a central HDAC interaction motif, a nuclear localization signal, and a C‑terminal Helix-Turn-Helix motif. Alternate splicing produces a truncated 406 aa variant. Within the region used as an immunogen, human LCoR shares 97% aa identity with mouse and rat LCoR.

Long Name

Ligand-dependent Nuclear Receptor Corepressor

Entrez Gene IDs

84458 (Human); 212391 (Mouse); 365462 (Rat)

Alternate Names

FLJ38026; KIAA1795RP11-175O19.1; LCoR; ligand dependent nuclear receptor corepressor; Mblk1-related protein 2; MLR2; MLR2ligand-dependent corepressor