Human Leptin R Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Leptin R

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-R_L) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-R_s) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-R_L transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-R_s transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R is encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype. Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R binds leptin with high affinity and is a potent leptin antagonist.

Product Datasheets

Product Specific Notices

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Related Research Areas

• Cytokine and Growth Factor Receptors on VSMC
• Cytokines and Receptors in Angiogenesis
• Endocrine Regulation of Lipid Metabolism
• Endothelial Cell Development Molecules
• Glucose Homeostasis
• IL-6 Family
• Metabolic Peptide Hormones and Regulators
• Metabolism
• Neurotransmitter Receptors
• Neurotransmitter Receptors, Transporters, and Ion Channels
• Neurotransmitters, Neuroactive Molecules and Associated Enzymes
• Nutrition and Appetite
• Obesity
• Receptors in the Jak/STAT Pathway
• Red Blood Cells (RBCs)