Human LILRA1/LILRB1 APC-conjugated Antibody

Name: Human LILRA1/LILRB1 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB30851A
Rating: 5 (1 reviews)

Catalog #: FAB30851A 1 Review Datasheet / COA / SDS

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FAB30851A

Detection of LILRA1/LILRB1 in Human Blood Monocytes by Flow Cytometry.

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Human LILRA1/LILRB1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRA1 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) LILRB1 is observed and no cross-reactivity rhILT3, 4, 5, 6, rhLIR-7 or -8 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 586326

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LILRA1
Pro17-Asn461
Accession # O75019

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LILRA1/LILRB1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human LILRA1/LILRB1 APC-conjugated Monoclonal Antibody (Catalog # FAB30851A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRA1/LILRB1

Leukocyte Immunoglobulin-like Receptor 6 (LIR-6), also called LILRA1 or CD85i, is a glycoprotein member of the LIR family of leukocyte Ig-like receptors. As an activating LIR, LIR-6 has a short cytoplasmic tail and a charged amino acid (aa) within the TM domain which interacts with FcR gamma. Both four (LIR-6a) and two (LIR-6b) Ig-like domain forms are expressed by monocytes and B cells. Human LIR-6 has no non-primate ortholog, but shows up to 80% aa identity with human ILT-1c and KIR-p91/PIR-B. A mouse homolog, gp49B2, has structural similarity but limited sequence identity.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 1

Alternate Names

CD85I; LILRA1/LILRB1; LIR6; LIR-6