Human LILRB1/CD85j/ILT2 Antibody Summary
Gly24-His458
Accession # Q8NHL6
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of LILRB1/CD85j/ILT2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20171, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LILRB1/CD85j/ILT2
The immunoglobulin-like transcript (ILT) family of activating and inhibitory type immunoreceptors are expressed on many leukocyte subsets and function in the regulation of immune responses (1‑3). This family was also named leukocyte Ig-like receptors (LIR) and monocyte/macrophage Ig-like receptors (MIR). ILTs share significant homology with killer cell Ig-like receptors (KIR). The ILT genes are located on human chromosome 19q13.4 in the leukocyte receptor complex, which also include the genes encoding KIRs (4). With the exception of ILT-6, which is a soluble molecule, all ILT family members are type I transmembrane proteins having two or four extracellular Ig-like domains (2, 3). One subset of the ILT receptors (referred to as subfamily B of the LIRs) has long cytoplasmic tails containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events by recruiting SH2-containing protein tyrosine phosphatase-1. Another subset of the ILT receptors (referred to as subfamily A of the LIRs) contains activating receptors with short cytoplasmic regions that lack signal transduction motifs. These receptors contain a basic arginine residue within their transmembrane domains, which allows association with Fc R gamma, an immunoreceptor tyrosine-based activation motif (ITAM)-bearing signal adapter protein (1‑3). ILT2, also known as LIR1, MIR7, and CD85j, is expressed on most monocytes, dendritic cells, and mature B cells (1‑3). It is also expressed on small percentages of T cells and NK cells. ILT2 has four extracellular Ig-like domains and three cytoplasmic ITIMs. It functions as an inhibitory receptor that prevents cellular activation. ILT2 has been shown to bind classical (HLA-A and -B) and nonclassical (HLA-G1, -E and -F) MHC class I molecules (MHCI) (1‑3). ILT2 also binds with high affinity to an MHC class I homologue from human cytomegalovirus (3). Ligation of ILT2 by MHC class I may function to poise cellular activation thresholds and inhibit various leukocyte effector mechanisms that are regulated by MHC class I molecules on target cells.
- Allen, D. et al. (2000) Immunobiol. 202:34.
- Colonna, M. et al. (1999) J. Leukocyte Biol. 66:375.
- Borges, L. and D. Cosman (2000) Cytokine Growth Factor Rev. 11:209.
- Young, N. et al. (2001) Immunogenetics 53:270.
Product Datasheets
Citations for Human LILRB1/CD85j/ILT2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Leukocyte Ig-Like receptor B1 restrains dendritic cell function through increased expression of the NF-?B regulator ABIN1/TNIP1
J Leukoc Biol, 2016-04-29;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
MiRNA-mediated control of HLA-G expression and function.
Authors: Manaster I, Goldman-Wohl D, Greenfield C
PLoS ONE, 2012-03-16;7(3):e33395.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Heat shock protein 27 differentiates tolerogenic macrophages that may support human breast cancer progression.
Authors: Banerjee S, Lin CF, Skinner KA, Schiffhauer LM, Peacock J, Hicks DG, Redmond EM, Morrow D, Huston A, Shayne M, Langstein HN, Miller-Graziano CL, Strickland J, O'Donoghue L, De AK
Cancer Res., 2011-01-11;71(2):318-27.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cytokine induced expression of programmed death ligands in human neutrophils.
Authors: Bankey PE, Banerjee S, Zucchiatti A, De M, Sleem RW, Lin CF, Miller-Graziano CL, De AK
Immunol. Lett., 2010-02-01;129(2):100-7.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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