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Human LILRB4/CD85k/ILT3 Biotinylated Antibody

Catalog #: BAF2425
4 Citations 1 Review Datasheet / COA / SDS
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BAF2425
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRB4/CD85k/ILT3 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LILRB4/CD85k/ILT3 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) ILT4 and rhILT5 and 5% cross-reactivity with rhILT2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRB4/CD85k/ILT3 Pro17-His257 Accession # ABM86208
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin
Purity
Antigen Affinity-purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human LILRB4/CD85k/ILT3
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood monocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: LILRB4/CD85k/ILT3

ILT3, also known as leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) and leukocyte immunoglobulin-like receptor 5 (LIR-5), is a type I membrane protein that functions as a receptor for class I MHC antigens. It contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses.

Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4
Entrez Gene IDs
11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)
Alternate Names
CD85 antigen-like family member K; CD85k antigen; CD85k; HM18; ILT3; ILT-3; ILT3CD85K; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB4; LIR5; LIR5LILRB5; LIR-5subfamily B, member 4; member 4

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Citations for Human LILRB4/CD85k/ILT3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Interferon beta and vitamin D synergize to induce immunoregulatory receptors on peripheral blood monocytes of multiple sclerosis patients.
    Authors: Waschbisch A, Sanderson N, Krumbholz M, Vlad G, Theil D, Schwab S, Maurer M, Derfuss T
    PLoS ONE, 2014-12-31;9(12):e115488.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  2. Cytokine induced expression of programmed death ligands in human neutrophils.
    Authors: Bankey PE, Banerjee S, Zucchiatti A, De M, Sleem RW, Lin CF, Miller-Graziano CL, De AK
    Immunol. Lett., 2010-02-01;129(2):100-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Inhibitory immunoglobulin-like receptors LILRB and PIR-B negatively regulate osteoclast development.
    Authors: Mori Y, Tsuji S, Inui M, Sakamoto Y, Endo S, Ito Y, Fujimura S, Koga T, Nakamura A, Takayanagi H, Itoi E, Takai T
    J. Immunol., 2008-10-01;181(7):4742-51.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Soluble Ig-like transcript 3 inhibits tumor allograft rejection in humanized SCID mice and T cell responses in cancer patients.
    Authors: Suciu-Foca N, Feirt N, Zhang QY, Vlad G, Liu Z, Lin H, Chang CC, Ho EK, Colovai AI, Kaufman H, D'Agati VD, Thaker HM, Remotti H, Galluzzo S, Cinti P, Rabitti C, Allendorf J, Chabot J, Caricato M, Coppola R, Berloco P, Cortesini R
    J. Immunol., 2007-06-01;178(11):7432-41.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human LILRB4/CD85k/ILT3 Biotinylated Antibody

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Human LILRB4/CD85k/ILT3 Biotinylated Antibody
By Anonymous on 07/07/2020
Application: ELISA Sample Tested: Serum and Plasma Species: Human
ELISA Human LILRB4/CD85k/ILT3 Biotinylated Antibody BAF2425