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Human LIN-28A Antibody

Catalog #: AF3757
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3757
AF3757-SP
Detection of Human LIN‑28A by Western Blot.
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Product Details
Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LIN-28A Antibody Summary

Species Reactivity
Human
Specificity
Detects human LIN-28A in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LIN-28A
Met1-Asn209
Accession # Q9H9Z2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human LIN-28A antibody by Western Blot. View Larger

Detection of Human LIN‑28A by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human LIN-28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LIN-28A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry LIN-28A antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

LIN‑28A in BG01V Human Stem Cells. LIN-28A was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human LIN-28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry LIN-28A antibody in D3 Mouse Stem Cells by Immunocytochemistry (ICC). View Larger

LIN‑28A in D3 Mouse Stem Cells. LIN-28A was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human LIN-28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western View Larger

Detection of Human LIN-28A by Simple WesternTM. Simple Western lane view shows lysates of NTera‑2 human testicular embryonic carcinoma cell line and JAR human choriocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LIN-28A at approximately 45 kDa (as indicated) using 10 µg/mL of Goat Anti-Human LIN-28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIN-28A

Human LIN-28 (Protein lin-28 homolog A; CSDD1, LIN28 and ZCCHC1)  is a 30 kDa (209 amino acids) cytoplasmic RNA-binding protein with an N-terminal cold shock domain and two C-terminal CCHC zinc finger domains. It is expressed by various undifferentiated embryonic cell types and is also present in adult cardiac and skeletal muscle. Expression of LIN-28 has been shown to be regulated by micro-RNA. Human LIN-28 shares 98% and 97% amino acid sequence homology with rat and mouse LIN-28, respectively.

Long Name
RNA-binding Protein LIN-28
Entrez Gene IDs
79727 (Human); 83557 (Mouse); 298542 (Rat)
Alternate Names
CSDD1; CSDD1lin-28A; FLJ12457; lin-28 homolog (C. elegans); lin-28 homolog A (C. elegans); LIN28; LIN-28; LIN28A; LIN-28A; LIN28RNA-binding protein LIN-28; Tex17; ZCCHC1; ZCCHC1protein lin-28 homolog A; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1

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Citations for Human LIN-28A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cells
    Authors: R Kitajima, R Nakai, T Imamura, T Kameda, D Kozuka, H Hirai, H Ito, H Imai, M Imamura
    Stem Cell Res, 2020-02-28;44(0):101749.
    Species: Pan troglodytes (Chimpanzee)
    Sample Types: Whole Cells
    Applications: ICC
  2. Exonuclease Domain-Containing 1 Enhances MIWI2 piRNA Biogenesis via Its Interaction with TDRD12
    Authors: RR Pandey, D Homolka, O Olotu, R Sachidanan, N Kotaja, RS Pillai
    Cell Rep, 2018-09-25;24(13):3423-3432.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata)
    Authors: R Nakai, M Ohnuki, K Kuroki, H Ito, H Hirai, R Kitajima, T Fujimoto, M Nakagawa, W Enard, M Imamura
    Sci Rep, 2018-08-15;8(1):12187.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: ICC
  4. Biallelic loss of human CTNNA2, encoding ?N-catenin, leads to ARP2/3 complex overactivity and disordered cortical neuronal migration
    Authors: AE Schaffer, MW Breuss, AO Caglayan, N Al-Sanaa, HY Al-Abdulwa, H Kaymakçala, C Y?lmaz, MS Zaki, RO Rosti, B Copeland, ST Baek, D Musaev, EC Scott, T Ben-Omran, A Karimineja, H Kayserili, F Mojahedi, M Kara, N Cai, JL Silhavy, S Elsharif, E Fenerciogl, BA Barshop, B Kara, R Wang, V Stanley, KN James, R Nachnani, A Kalur, H Megahed, F Incecik, S Danda, Y Alanay, E Faqeih, G Melikishvi, L Mansour, I Miller, B Sukhudyan, J Chelly, WB Dobyns, K Bilguvar, RA Jamra, M Gunel, JG Gleeson
    Nat. Genet., 2018-07-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Autologous and Heterologous Cell Therapy for Hemophilia B toward Functional Restoration of Factor IX
    Authors: S Ramaswamy, N Tonnu, T Menon, BM Lewis, KT Green, D Wampler, PE Monahan, IM Verma
    Cell Rep, 2018-05-01;23(5):1565-1580.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Embryonic lethality and defective male germ cell development in mice lacking UTF1
    Authors: SD Kasowitz, M Luo, J Ma, NA Leu, PJ Wang
    Sci Rep, 2017-12-08;7(1):17259.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate
    Authors: T Shibata, Y Fujita, H Ohno, Y Suzuki, K Hayashi, KR Komatsu, S Kawasaki, K Hidaka, S Yonehara, H Sugiyama, M Endo, H Saito
    Nat Commun, 2017-09-14;8(1):540.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Glycolysis-Optimized Conditions Enhance Maintenance of Regenerative Integrity in Mouse Spermatogonial Stem Cells during Long-Term Culture
    Authors: AR Helsel, MJ Oatley, JM Oatley
    Stem Cell Reports, 2017-04-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture.
    Authors: Makela J, Toppari J, Rivero-Muller A, Ventela S
    PLoS ONE, 2014-03-11;9(3):e90088.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach.
    Authors: Plews JR, Li J, Jones M, Moore HD, Mason C, Andrews PW, Na J
    PLoS ONE, 2010-12-30;5(12):e14397.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
    Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S
    Cell, 2007-11-30;131(5):861-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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