Human LRRC52 Antibody Summary
Ser24-Asp244
Accession # Q8N7C0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of LRRC52 in HEK293 Human Cell Line Transfected with Human LRRC52 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with (A) human LRRC52 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human LRRC52 Monoclonal Antibody (Catalog # MAB10260) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRRC52
LRRC52 (Leucine-rich repeat-containing protein 52) belongs to an extracellular leucine-rich-repeat–only (Elron) cluster, which includes five other LRRC proteins: LRRC26, LRRC38, LRRC55, LRTM1 and LRTM2 (1). Elron cluster members are type I transmembrane proteins with an extracellular domain (ECD) containing several LRR motifs and a cytoplasmic C-terminal tail with a short stretch of acidic residues (1). The mature ECD of LRRC52 is 221 amino acids (aa), containing 5 LRR motifs, and shares 76% and 78% identity with mouse and rat LRRC52, respectively. LRRC-52 is dominantly expressed in testis and skeletal muscle and is also detected in several other tissues including placenta, kidney, lung, and some glands (2, 3). LRRC52 has been identified as a testes specific SLO3 K+ channel accessory protein in mouse (2). LRRC52 is proposed to be essential for alkalization dependent activation of the KSper current at physiological membrane potentials and pH (2). Activation of the SLO3 channel is critical in the fertilization process and loss of function results in infertility (2, 4, 5). A second Elron cluster protein, LRRC26, was recently identified as a regulatory subunit of SLO1 BK-type K+ channels, suggesting a general function for the entire family (6). Based on function, LRRC52 might be a therapeutic target for male contraception (7).
- Dolan, J. et al. (2007) BMC Genomics 8:320.
- Yang, C. et al. (2011) PNAS 108:19419.
- Leonetti, M. et al. (2012) PNAS. 109:19274.
- Navarro, B. et al. (2007) PNAS 104:7688.
- Sanit, C. et al. (2010) FEBS Lett. 584:1041.
- Yan, J. and Aldrich, RW. (2010) Nature 466:513.
- Zeng, XH. et al. (2015) PNAS 112:2599.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human LRRC52 Antibody
There are currently no reviews for this product. Be the first to review Human LRRC52 Antibody and earn rewards!
Have you used Human LRRC52 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image