Human Lysine (K)-specific Demethylase 6B/KDM6B Antibody

Catalog #: AF7300 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7300
AF7300-SP

Lysine (K)-specific Demethylase 6B/KDM6B in BG01V Human Stem Cells.

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All Lysine (K)-specific Demethylase 6B/KDM6B Antibodies

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Human Lysine (K)-specific Demethylase 6B/KDM6B Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lysine (K)-specific Demethylase 6B/KDM6B in direct ELISAs.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Lysine (K)-specific Demethylase 6B/KDM6B (isoform 1)
Pro1530-Arg1682
Accession # NP_001073893

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Lysine (K)-specific Demethylase 6B/KDM6B in BG01V Human Stem Cells. Lysine (K)-specific Demethylase 6B/KDM6B was detected in immersion fixed BG01V human embryonic stem cells, differentiated (upper panel) and undifferentiated (lower panel) using Goat Anti-Human Lysine (K)-specific Demethylase 6B/KDM6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7300) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lysine (K)-specific Demethylase 6B/KDM6B

KDM6B (Lysine [K]-specific demethylase 6B; also JMJD3) is a 170-185 kDa member of the UTX family of enzymes. It is expressed in both embryo and adult, and specifically demethylates tri- and di-methylated Lys27 on histone H3 (H3K27). Methylation of H3K27 by PRC2 inactivates genes, while demethylation of methylated H3K27 by KDM6B activates genes. Human KDM6B is 1643 amino acids (aa) in length. It contains two Pro-rich regions (aa 32-85 and 1046-1082) plus one jumonji domain (aa 1339-1502). One potential splice variant is reported that contains a 39 aa insertion after Leu1636. Over aa 1530-1682, which is a sequence that contains the 39 aa insertion, human KDM6B shares 75% aa sequence identity with mouse KDM6B. When the insertion is disregarded, human and mouse KDM6B are identical in aa sequence over the above range of amino acids.

Entrez Gene IDs

23135 (Human); 216850 (Mouse); 363630 (Rat)

Alternate Names

EC 1.14.11; EC 1.14.11.-; JmjC domain-containing protein 3; JMJD3; JMJD3lysine-specific demethylase 6B; jumonji domain containing 3, histone lysine demethylase; Jumonji domain-containing protein 3; KDM6B; KIAA0346jumonji domain containing 3; Lysine (K)specific Demethylase 6B; Lysine (K)-specific Demethylase 6B; Lysine demethylase 6B