Human MAdCAM-1 Antibody

Catalog #: AF6056 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6056-100
AF6056-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MAdCAM-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MADCAM-1 in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MADCAM-1
Met1-Leu333
Accession # Q13477

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human MAdCAM‑1 Monoclonal Antibody (Catalog # MAB60561).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human MAdCAM-1 DuoSet ELISA Kit (Catalog # DY6056-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human MAdCAM‑1 ELISA Standard Curve. Recombinant Human MAdCAM-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human MAdCAM-1 Monoclonal Antibody (Catalog # MAB60561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6056) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAdCAM-1

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an approximately 60 kDa type 1 transmembrane glycoprotein. It is an endothelial cell adhesion molecule that belongs to the immunoglobulin (Ig) superfamily of proteins (1). Human MAdCAM-1 is synthesized as a 382 amino acid (aa) precursor that contains an 18 aa signal sequence, a 299 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD there is one potential site for N-linked glycosylation (2). The ECD comprises two Ig-like domains of 90 aa and 119 aa, respectively, each possessing invariant cysteine residues that stabilize the Ig loop (2). There is also a Ser-Thr-Pro-rich (71%) mucin-like 48 aa domain that is (aa 206‑317) formed by six tandem repeats of an eight aa sequence having the general consensus DTTSPEP/SP. This mucin domain contains 19 potential sites for O-linked glycosylation (2, 3). A splicing variant in which a single Ala residue is substituted for aa 223‑334 in isoform 1 produces a second isoform. Human mature MAdCAM-1 shares only 44% aa sequence identity with mature mouse MAdCAM-1. The integrin alpha (4) beta (7), which is expressed on lymphocytes, functions as the MAdCAM-1 receptor (1). The Ig domains of MAdCAM-1 are critical to alpha (4) beta (7) binding, and the mucin domain has activity in L‑Selectin binding. MAdCAM-1 expression is up-regulated by TNF-alpha and IL‑1 beta. MAdCAM-1 is expressed on the surface of high endothelial venules (HEV) in the gut and in Peyer’s patches, on endothelial cells of the mesenteric lymph nodes, lamina propria of the small and large intestine, and the mammary gland during lactation, and on brain endothelial cells (1). MAdCAM‑1 has also been reported to be expressed in the liver portal region in autoimmune hepatitis (1), and in bone marrow following allogenic (genetically non-identical) hematopoietic stem cell transplantation, where it recruits donor T cells, which may lead to graft versus host disease (3, 4). MAdCAM‑1 functions as a homing receptor, and plays a central role in leukocyte migration into HEVs and Peyer’s patch (5). In addition to its normal role in lymphocyte trafficking to mucosal tissue, MAdCAM‑1 expression is also dramatically increased in chronic inflammatory and disease states (1, 6), including inflammatory bowel disease (Crohn’s disease and ulcerative colitis) (7), sclerosing cholangitis (8), and diabetes (9), and may play an important role in these conditions.

References

Ando, T. et al. (2007) BMC Physiol. 7:10.
Dando, J. et al. (2002) Acta Crystallogr. D 58:233.
Leung, E. et al. (1996) Immunol. Cell Biol. 74:490.
Ambruzova, Z. et al. (2009) Hum. Immunol. 70:457.
Tada, T. et al. (2008) Exp. Anim. 57:247.
Volpes, R. et al. (1992) Hepatology 15:269.
Connor, E.M. et al. (1999) J. Leukoc. Biol. 65:349.
Ala, A. et al. (2001) Gut 49:3043.
Yang, X.D. et al. (1997) Diabetes 46:1542.

Long Name

Mucosal Addressin Cell Adhesion Molecule 1

Entrez Gene IDs

8174 (Human); 17123 (Mouse)

Alternate Names

hMAdCAM-1; MACAM1; MAdCAM1; MAdCAM-1; mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1