Human MAGP-2/MFAP5 Antibody

Catalog #: AF4914 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4914
AF4914-SP

All MAGP-2/MFAP5 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MAGP-2/MFAP5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MAGP-2/MFAP5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human MAGP-1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MAGP-2/MFAP5
Ile22-Leu173
Accession # Q13361

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human MAGP-2/MFAP5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAGP-2/MFAP5

MAGP-2 (Microfibril-associated glycoprotein 2; also MFAP-5) is a 25 kDa member of the MFAP family of proteins. It is secreted by smooth muscle cells and has multiple functions. Through its binding to EGF repeats, MAGP-2 mediates the release of Jagged1 and Notch extracellular domains, and also binds to matrix microfibrillar proteins, inducing elastin fiber formation. Mature human MAGP-2 is 152 amino acids (aa) in length. It contains an RDG motif (aa 30‑32) and a central Cys-rich domain (aa 84‑140) that participates in covalent binding to fibrillin microfibrils. One potential splice variant exists that shows a 10 aa deletion between aa 73‑82. Mature human MAGP-2 is 86% and 87% aa identical to mouse and canine MAGP-2, respectively.

Long Name

Microfibril-associated Glycoprotein 2

Entrez Gene IDs

8076 (Human); 50530 (Mouse); 362429 (Rat)

Alternate Names

MAGP2; MAGP-2; MAGP2MFAP-5; MFAP5; Microfibril-associated glycoprotein 2; microfibrillar associated protein 5; microfibrillar-associated protein 5; MP25; MP25microfibril-associated glycoprotein-2