Human MCAM/CD146 Fluorescein-conjugated Antibody

Catalog #: FAB932F
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB932F-100
FAB932F-025

Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry.

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All MCAM/CD146 Antibodies

Product Details

Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MCAM/CD146 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm)OCAM, or rmMAdCAM-1.

Source

Monoclonal Mouse IgG₁ Clone # 128018

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MCAM/CD146
Val24-Gly559
Accession # AAA20824

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MCAM/CD146 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB932F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MCAM/CD146

MCAM, also known as CD146 and MUC18, is a putative adhesion molecule that belongs to the immunoglobulin superfamily. It is associated with tumor progression and metastasis of various carcinomas and may be involved in embryonic neural development.

Long Name

Melanoma Cell Adhesion Molecule

Entrez Gene IDs

4162 (Human); 84004 (Mouse); 78967 (Rat)

Alternate Names

CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human MCAM/CD146 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
Authors: S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis
Stem Cell Res Ther, 2019-11-04;10(1):316.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
PLoS ONE, 2014-01-29;9(1):e84417.
Late outgrowth endothelial cells resemble mature endothelial cells and are not derived from bone marrow.
Authors: Tura O, Skinner E, Barclay G, Samuel K, Gallagher R, Brittan M, Hadoke P, Newby D, Turner M, Mills N
Stem Cells, 2013-02-01;31(2):338-48.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Oxygen tension modifies the 'stemness' of human cord blood-derived stem cells.
Authors: Buchheiser A, Houben A, Bosch J, Marbach J, Liedtke S, Kogler G
Cytotherapy, 2012-04-12;14(8):967-82.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway.
Authors: Liu N, Shi S, Deng M, Tang L, Zhang G, Liu N, Ding B, Liu W, Liu Y, Shi H, Liu L, Jin Y
J. Bone Miner. Res., 2011-09-01;26(9):2082-95.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Hyperoxia disrupts vascular endothelial growth factor-nitric oxide signaling and decreases growth of endothelial colony-forming cells from preterm infants.
Authors: Fujinaga H, Baker CD, Ryan SL, Markham NE, Seedorf GJ, Balasubramaniam V, Abman SH
Am. J. Physiol. Lung Cell Mol. Physiol., 2009-09-04;297(6):L1160-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry