Human MCEMP1 Antibody

Catalog #: AF4994 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4994
AF4994-SP

All MCEMP1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MCEMP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MCEMP1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MCEMP1
Val106-Gln187
Accession # Q8IX19

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human MCEMP1

Flow Cytometry

0.25 µg/10⁶ cells

Human whole blood and THP‑1 human acute monocytic leukemia cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCEMP1

MCEMP1 (mast cell-expressed membrane protein 1; also C19orf59) is a 22 kDa mast cell specific protein expressed in lung. Human MCEMP1 is 187 amino acids (aa) in length. It is a type II transmembrane protein that contains an 85 aa N-terminal cytoplasmic region and an 81 aa C-terminal extracellular domain (aa 107‑187). There is one potential splice variant that shows a 22 aa substitution for the C-terminal five amino acids. Over aa 106‑187, human MCEMP1 shares only 52% aa identity with the presumed mouse MCEMP1 ortholog.

Long Name

Mast Cell-expressed Membrane Protein 1

Entrez Gene IDs

199675 (Human); 69189 (Mouse); 498128 (Rat)

Alternate Names

C19orf59; chromosome 19 open reading frame 59; mast cell-expressed membrane protein 1; MCEMP1; MGC132456