Human MDGA1 Antibody

Catalog #: AF5055 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5055
AF5055-SP

All MDGA1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MDGA1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MDGA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human MDGA2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MDGA1
Gln19-Gly931
Accession # Q8NFP4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human MDGA1 (Catalog # 5055-MD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MDGA1

MDGA1 (MAM domain containing glycosylphosphatidylinositol anchor 1; also named GPIM) is a 140 kDa member of the Ig superfamily of proteins (1). Human MDGA1 is synthesized as a 955 amino acid (aa) precursor that contains an 18 aa signal sequence, a 914 aa mature chain, and a 23 aa propeptide. The mature chain consists of six Ig-like domains, a fibronectin type III domain (aa 640‑739), a MAM domain (aa 751‑918), 11 potential sites for N-linked glycosylation, and a GPI anchor (aa 932) (1‑3). Alternative splicing in human MDGA1 produces an isoform variant that shows a 48 aa substitution for aa corresponding to positions 926‑955. Mature human MDGA1 shares 96%, 94%, and 80% aa sequence identity with mature rat, mouse, and chick MDGA1, respectively. MDGA1 is structurally similar to other IgCAMS, such as the L1 family and axonin 1, which have roles in cell adhesion, migration, and process outgrowth (4). Expression analysis in normal human tissues reveals that MDGA1 is expressed as a 5 kb and 9.5 kb mRNA (2). The smaller transcript is highly expressed in some human cancer cell lines, as well as in different primary tumors (lung, colon, uterus, stomach, and breast) (2). Gene expression is higher in several tumor tissues as compared with corresponding normal tissues, and may be involved in tumor progression (2). The 9.5 kb transcript is found exclusively in the central and peripheral nervous system (1‑2, 4). One study shows that MGDA1 is expressed in mice neocortex by layer 2/3 neurons throughout their development (4), and it is involved in controlling the migration and lamination of layer 2/3 neurons (5).

References