Human Megalin/LRP2 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB9578P-100
Detection of Megalin/LRP2 in NSO cells transfected with Human LRP2 cells by Flow Cytometry.
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Human Megalin/LRP2 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Megalin/LRP2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 545606
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Megalin/LRP2
Pro3510-Lys3964
Accession # P98164
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
NSO cells transfected with Human LRP2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry View Larger

Detection of Megalin/LRP2 in NSO cells transfected with Human LRP2 cells by Flow Cytometry. NSO cells transfected with Human LRP2 were stained with Mouse Anti-Human Megalin/LRP2 PE‑conjugated Monoclonal Antibody (Catalog # FAB9578P, filled histogram) or irrelevant transfectant. To facilitate intracellular staining, cells were fixed with FC004 and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Megalin/LRP2

Megalin, also known as the low-density lipoprotein receptor-related protein 2 (LRP2), is a large type I transmembrane cell surface protein. This glycoprotein is a multi‑ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney (1). The Megalin protein is critical for the re-uptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling. Mutations in this gene cause Donnai-Barrow Syndrome (DBS) and Facio-Oculoacoustico-Renal Syndrome (FOAR) (1). Megalin is consisting of a 25 amino acid (aa) probable N‑terminal signal peptide sequence, a 4400 aa extracellular region, a 22 aa single transmembrane domain, and a 213 aa C‑terminal cytoplasmic tail. The entire extracellular region is made up of 36 class A motifs of putative ligand-binding domains arranged in four distinct clusters, 16 growth factor repeats separated by 8 YWTD spacer regions, and 1 epidermal growth factor-like repeat (2). The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase (3). The amino acid 3510-3964 encodes the fourth class A motif cluster in human Megalin, termed Megalin C4. Human Megalin C4 shares 77% and 74% identity with mouse and rat Megalin C4.

References
  1. Christensen, E. I. and Birn, H. (2002) Nat. Rev. Mol. Cell Biol 3:256.
  2. Saito, A. et al. (1994) Proc.Natl. Aca. Sci. U. S. A. 91:9725.
  3. Kantarci, S. et al. (2007) Nat. Genet 39:957.
Long Name
LDL Receptor-related Protein 2
Entrez Gene IDs
4036 (Human); 14725 (Mouse); 29216 (Rat)
Alternate Names
calcium sensor protein; DBS; DBSlow-density lipoprotein receptor-related protein 2; EC 1.1.2.3; EC 3.4.21.9; Glycoprotein 330; gp330; Heymann nephritis antigen homolog; low density lipoprotein receptor-related protein 2; LRP2; LRP-2; Megalin

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