Human MEK2 Antibody

Catalog #: AF2855 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2855
AF2855-SP

Detection of Human MEK2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MEK2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MEK2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MEK2
Met1-Val400
Accession # P36507

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MEK2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human MEK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2855) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For add-itional reference, recombinant human MEK1 and Recombinant Human Active MEK2 (Catalog # 2855-KS) (10 ng/lane) were included. A specific band for MEK2 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEK2

Mitogen-activated protein kinase kinase 2 (MAP2K2), also known as MEK2 and MKK2, belongs to the STE grouping of kinases. Both MEK2 and the related MEK1 are dual-specificity kinases, phosphorylating and activating the mitogen-activated protein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. Activation of MEK2 by Raf occurs through phosphorylation at S222 and S226.

Long Name

Mitogen-activated Protein Kinase Kinase 2

Entrez Gene IDs

5605 (Human); 26396 (Mouse)

Alternate Names

ERK activator kinase 2; MAP2K2; MAPKK 2; MEK 2; MEK2; MEK2EC 2.7.12.2; mitogen-activated protein kinase kinase 2; MKK2; MKK2FLJ26075; p45; PRKMK2MAPKK2