Human Methionine Aminopeptidase 1/
METAP1 Antibody

Catalog #: AF3537 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3537
AF3537-SP

All Methionine Aminopeptidase 1/METAP1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Methionine Aminopeptidase 1/
METAP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Methionine Aminopeptidase 1/METAP1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Methionine Aminopeptidase 1/METAP1
His52-Phe386
Accession # P53582

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Methionine Aminopeptidase 1 (Catalog # 3537-ZN)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Methionine Aminopeptidase 1 (Catalog # 3537-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Methionine Aminopeptidase 1/METAP1

The human METAP1 gene encodes Methionine Aminopeptidase 1, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). Inhibition of METAPs provides a novel strategy in developing anti-cancer drugs (3). The purified rhMETAP1 consists of amino acid residues 52/53 to 386. A previous report showed that the N-terminal 89 amino acid region was not essential for catalytic activity (4).

References

Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta. 1477:157.
Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
Liu, S. et al. (1998) Science 282:1324.
Addlagatta, A. et al. (2005) Biochemistry 44:14741.

Entrez Gene IDs

23173 (Human); 75624 (Mouse)

Alternate Names

DKFZp781C0419; EC 3.4.11.18; KIAA0094MAP 1; MAP1; MAP1A; MetAP 1; MetAP1; MetAP1A; Methionine Aminopeptidase 1; methionyl aminopeptidase 1; Peptidase M 1