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Human MICA/B Antibody

Catalog #: MAB13001
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB13001-SP
MAB13001-500
MAB13001-100
Detection of MICA/B in K562 Human Cell Line by Flow Cytometry.
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Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MICA/B Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICA/B in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 159207
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human MICA
Accession # Q29983
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.03-0.1 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Biotinylated Human MICA Fc Chimera to immobilized Recombinant Human NKG2D Fc Chimera (Catalog # 1299‑NK) coated at 2 µg/mL (100 µL/well). At 0.4 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MICA/B antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MICA/B in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA/B Monoclonal Antibody (Catalog # MAB13001, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry MICA/B antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

MICA/B in Human Pancreas. MICA/B was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human MICA/B Monoclonal Antibody (Catalog # MAB13001) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICA/B

MICA and MICB are stress-induced type I transmembrane proteins that function as ligands for NKG2D. They are distantly related to MHC class I proteins and are expressed on epithelial tumors and in response to infections.

Long Name
MHC Class I-related Protein A and B
Entrez Gene IDs
4276 (Human)
Alternate Names
MHC class I chain-related protein A; MHC class I chain-related protein B; MIC; MICA; MICA/B; MICB; PERB11; PERB11.1; PERB11.2

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Citations for Human MICA/B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Exploiting the DNA Damaging Activity of Liposomal Low Dose Cytarabine for Cancer Immunotherapy
    Authors: JD Lewicky, AL Martel, NL Fraleigh, E Picard, L Mousavifar, A Nakamura, F Diaz-Mitom, R Roy, HT Le
    Pharmaceutics, 2022-12-03;14(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The metabolism of cells regulates their sensitivity to NK cells depending on p53 status
    Authors: S Belkahla, JM Brualla, A Fayd'herbe, P Falvo, N Allende-Ve, M Constantin, AUH Khan, L Coenon, C Alexia, G Mitola, P Massa, S Orecchioni, F Bertolini, W Mnif, J Hernandez, A Anel, M Villalba
    Scientific Reports, 2022-02-25;12(1):3234.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1)
    Authors: N Allende-Ve, J Marco Brua, P Falvo, C Alexia, M Constantin, AF de Maudave, L Coenon, D Gitenay, G Mitola, P Massa, S Orecchioni, F Bertolini, I Marzo, A Anel, M Villalba
    Scientific Reports, 2022-01-25;12(1):1341.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  4. Toll-like Receptor-4 Activation Boosts the Immunosuppressive Properties of Tumor Cells-derived Exosomes
    Authors: R Domenis, A Cifù, D Marinò, M Fabris, KR Niazi, P Soon-Shion, F Curcio
    Sci Rep, 2019-06-11;9(1):8457.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Lower protein expression levels of MHC class I chain-related gene A in hepatocellular carcinoma are at high risk of recurrence after surgical resection
    Authors: CF Huang, SC Wang, WT Chang, ML Yeh, CI Huang, ZY Lin, SC Chen, WL Chuang, JF Huang, CY Dai, YL Chen, ML Yu
    Sci Rep, 2018-10-25;8(1):15821.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
    Authors: Cox S, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal J, Saudemont A
    Eur J Immunol, 2015-06-09;45(8):2324-34.
    Species: Human
    Sample Types: Plasma
    Applications: Immunoprecipitation
  7. Hepatitis C virus impairs natural killer cell-mediated augmentation of complement synthesis.
    Authors: Kim H, Bose S, Meyer K, Ray R
    J Virol, 2013-12-18;88(5):2564-71.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
    Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S
    PLoS Pathog., 2012-01-12;8(1):e1002486.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
    Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
    PLoS ONE, 2011-02-25;6(2):e16899.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  10. Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
    Authors: Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
    Oncogene, 2011-01-24;30(23):2622-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Transfer of NKG2D and MICB at the cytotoxic NK cell immune synapse correlates with a reduction in NK cell cytotoxic function.
    Authors: Roda-Navarro P, Vales-Gomez M, Chisholm SE, Reyburn HT
    Proc. Natl. Acad. Sci. U.S.A., 2006-07-18;103(30):11258-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B.
    Authors: Skov S, Pedersen MT, Andresen L, Straten PT, Woetmann A, Odum N
    Cancer Res., 2005-12-01;65(23):11136-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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