Human/Mouse B7-H4 Antibody

Catalog #: AF2154
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2154
AF2154-SP

Detection of Mouse and Human B7‑H4 by Western Blot.

2 Images

All B7-H4 Antibodies

Product Details

Citations (4)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse B7-H4 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse B7-H4 in direct ELISAs and Western blots. Detects human and mouse B7-H4 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse B7-H4
Phe29-Pro258
Accession # Q7TSP5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Mouse and Human B7‑H4 by Western Blot. Western blot shows lysates of mouse placenta tissue, mouse uterus tissue, and SK-BR-3 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse B7-H4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for B7-H4 at approximately 50-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

View Larger

Detection of B7-H4 in HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H4 or (B) irrelevant protein and eGFP was stained with Goat Anti-Human/Mouse B7-H4 Polyclonal Antibody (Catalog # AF2154) followed by APC-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0108). Quadrant markers were set based on Goat IgG control antibody staining (Catalog # AB-108-C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H4

B7-H4, also known as B7x and B7S1, is a 50 ‑ 80 kDa glycosylated member of the B7 family of immune co‑stimulatory proteins (1, 2). Mature mouse B7-H4 consists of a 230 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain which is followed by a hydrophobic C-terminal region (3 - 5). Within the ECD, mouse B7-H4 shares 90% and 99% aa sequence identity with human and rat B7-H4, respectively. It shares 21% ‑ 29% aa sequence identity with mouse B7-1, B7-2, B7-H1, B7-H2, B7‑H3, and PD-L2. B7-H4 is expressed on the surface of activated lymphocytes, macrophages, monocytes, dendritic cells, epithelial cells, and bone marrow-derived mesenchymal stem cells (4 - 8). Its binding to activated T cells dampens T cell responses and induces cell cycle arrest in the T cell (3 - 5). Reverse signaling can induce either cell cycle arrest or apoptosis in the B7-H4 expressing cell (9, 10). B7-H4 is up‑regulated in several carcinomas in correlation with tumor progression and metastasis (2, 7, 11, 12). A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status (13 - 15). Soluble B7-H4 functions as a decoy molecule that blocks the inhibitory influence of B7-H4 on immune activation (15). Despite evidence for the involvement of B7-H4 in immune regulation, mice deficient in its expression do not show significant immune deficiencies, suggesting compensation by other molecules in vivo (16).

References

Yi, K.H. and L. Chen (2009) Immunol. Rev. 229:145.
Salceda, S. et al. (2005) Exp. Cell Res. 306:128.
Zang, X. et al. (2003) Proc. Natl. Acad. Sci. 100:10388.
Prasad, V.R. et al. (2003) Immunity 18:863.
Sica, G.L. et al. (2003) Immunity 18:849.
Kryczek, I. et al. (2006) J. Exp. Med. 203:871.
Tringler, B. et al. (2005) Clin. Cancer Res. 11:1842.
Xue, Q. et al. (2010) Stem Cells Dev. 19:27.
Song, H. et al. (2008) Cancer Lett. 266:227.
Park, G.B. et al. (2009) Immunology 128:360.
Zang, X. et al. (2007) Proc. Natl. Acad. Sci. 104:19458.
Krambeck, A.E. et al. (2006) Proc. Natl. Acad. Sci. 103:10391.
Simon, I. et al. (2006) Cancer Res. 66:1570.
Thompson, R.H. et al. (2008) Cancer Res. 68:6054.
Azuma, T. et al. (2009) PloS Med. 6:e1000166.
Suh, W.-K. et al. (2006) Mol. Cell. Biol. 26:6403.

Long Name

B7 Homolog 4

Entrez Gene IDs

79679 (Human); 242122 (Mouse); 295322 (Rat)

Alternate Names

B7h.5; B7H4; B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1; B7S1VCTN1; B7x; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; Vtcn1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse B7-H4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

Pancreatic epithelial IL-17/IL-17RA signaling drives B7-H4 expression to promote tumorigenesis
Authors: Castro-Pando, S;Howell, RM;Li, L;Mascaro, M;Faraoni, EY;Le Roux, O;Romanin, D;Tahan, V;Riquelme, E;Zhang, Y;Kolls, JK;Allison, JP;Lozano, G;Moghaddam, SJ;McAllister, F;
Cancer immunology research
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry
Molecular Pathways: Evaluating the Potential for B7-H4 as an Immunoregulatory Target
Authors: HL MacGregor, PS Ohashi
Clin. Cancer Res, 2017-03-21;0(0):.
Applications: IHC
Loss of Peripheral Protection in Pancreatic Islets by Proteolysis-Driven Impairment of VTCN1 (B7-H4) Presentation Is Associated with the Development of Autoimmune Diabetes.
Authors: Radichev I, Maneva-Radicheva L, Amatya C, Salehi M, Parker C, Ellefson J, Burn P, Savinov A
J Immunol, 2016-01-15;196(4):1495-506.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
B7-H3 and B7x are highly expressed in human prostate cancer and associated with disease spread and poor outcome.
Authors: Zang X, Thompson RH, Al-Ahmadie HA, Serio AM, Reuter VE, Eastham JA, Scardino PT, Sharma P, Allison JP
Proc. Natl. Acad. Sci. U.S.A., 2007-11-27;104(49):19458-63.
Species: Human
Sample Types: Whole Tissue
Applications: IHC