Human/Mouse Bad aa 1‑21 Antibody

Name: Human/Mouse Bad aa 1‑21 Antibody
Brand: R&D Systems
SKU: AF819
Rating: 4 (2 reviews)

Catalog #: AF819
3 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF819
AF819-SP

Detection of Human/Mouse Bad by Western Blot.

2 Images

All Bad Antibodies

Product Details

Citations (3)

FAQs

Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Bad aa 1‑21 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Bad in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

KLH-coupled human Bad synthetic peptide
MFQIPEFEPSEQEDSSSAERGC
Accession # Q92934

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

0.3-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human/Mouse Bad by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse Bad Antigen Affinity-purified Polyclonal Antibody (Catalog # AF819) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bad at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry

View Larger

Bad in Jurkat Human Cell Line. Bad was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Rabbit Anti-Human/Mouse Bad Antigen Affinity-purified Polyclonal Antibody (Catalog # AF819) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bad

Bcl-2 antagonist of cell death (BAD) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of BAD on Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Over amino acid 1-21, human BAD shares 81 % aa sequence identity with mouse and rat BAD.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Entrez Gene IDs

572 (Human); 12015 (Mouse); 64639 (Rat)

Alternate Names

Bad; BBC2; BBC6; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; Bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; Bcl2-L-8; BCL2L8bcl2-L-8; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse Bad aa 1‑21 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

TCDD promotes lung tumors via attenuation of apoptosis through activation of the Akt and ERK1/2 signaling pathways.
Authors: Chen R, Siao S, Hsu C, Chang C, Chang L, Wu C, Lin P, Wang Y
PLoS ONE, 2014-06-13;9(6):e99586.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay
Sustained early growth response gene 3 expression inhibits the survival of CD4/CD8 double-positive thymocytes.
Authors: Xi H, Kersh GJ
J. Immunol., 2004-07-01;173(1):340-8.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
Translocation of full-length Bid to mitochondria during anoikis.
Authors: Valentijn AJ, Gilmore AP
J. Biol. Chem., 2004-05-17;279(31):32848-57.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot