Human/Mouse Bcl-w Antibody Summary
Ala2-Thr172
Accession # Q92843
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Bcl‑w antibody in Human Spleen by Immunohistochemistry (IHC-P). Bcl‑w antibody in Human Spleen by Immunohistochemistry (IHC-P).](https://resources.rndsystems.com/images/datasheets/antibody/Bcl-w_AF8241_Immunohistochemistry_7092.jpg)
Bcl‑w in Human Spleen. Bcl‑w was detected in immersion fixed paraffin-embedded sections of human spleen using 3 µg/mL Goat Anti-Human/Mouse Bcl‑w Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8241) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-w
Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
- Gross, A. et al. (1999) Genes and Develop. 13:1899.
- Kroemer, G. (1997) Nature Med. 3:614.
- Ross, J.A., et al. (1998) Nat. Genet. 18:251.
-
Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
-
Yan, W. et al. (2000) Mol. Endocrin. 14:682.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse Bcl-w Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse Bcl-w Antibody and earn rewards!
Have you used Human/Mouse Bcl-w Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image