Human/Mouse BID Antibody Summary
Met1-Asp195
Accession # P70444
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse BID by Western Blot. Western blot shows lysates of Recombinant Mouse BID and Recombinant Human BID untreated (-) or treated (+) with Caspase-8. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands for BID were detected at approximately 12 kDa and 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
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Detection of Human and Mouse BID by Western Blot. Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for BID at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BID
BID is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. BID is cytosolic in healthy cells but upon delivery of an apoptotic signal, BID is cleaved by Caspase-8. The cleaved form is translocated to the mitochondria outer membrane where it binds to BAK and the resulting complex causes altered mitochondrial membrane permeability.
Product Datasheets
Citations for Human/Mouse BID Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
8
Citations: Showing 1 - 8
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Apoptotic signaling clears engineered Salmonella in an organ-specific manner
Authors: Abele, TJ;Billman, ZP;Li, L;Harvest, CK;Bryan, AK;Magalski, GR;Lopez, JP;Larson, HN;Yin, XM;Miao, EA;
bioRxiv : the preprint server for biology
Species: Mouse, Transgenic Mouse
Sample Types: Cell Fraction, Cell Lysates
Applications: Western Blot -
LPS-induced inflammation desensitizes hepatocytes to Fas-induced apoptosis through Stat3 activation-The effect can be reversed by ruxolitinib
Authors: A Markotic, D Flegar, D Grcevic, A Sucur, H Lalic, P Turcic, N Kovacic, N Lukac, D Pravdic, K Vukojevic, I Cavar, T Kelava
J. Cell. Mol. Med., 2020-02-05;0(0):.
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot -
Impact of caspase-1/11, -3, -7, or IL-1?/IL-18 deficiency on rabies virus-induced macrophage cell death and onset of disease
Authors: E Kip, F Nazé, V Suin, T Vanden Ber, A Francart, S Lamoral, P Vandenabee, R Beyaert, S Van Gucht, M Kalai
Cell Death Discov, 2017-03-06;3(0):17012.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection.
Authors: Zhu, Wenhan, Tao, Lili, Quick, Marsha L, Joyce, Johanna, Qu, Jie-Ming, Luo, Zhao-Qin
PLoS Pathog, 2015-03-04;11(3):e1004704.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo.
Authors: Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C
Cell Death Differ, 2014-06-06;21(9):1442-50.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
BH3-only protein Bid is dispensable for seizure-induced neuronal death and the associated nuclear accumulation of apoptosis-inducing factor.
Authors: Engel T, Caballero-Caballero A, Schindler CK, Plesnila N, Strasser A, Prehn JH, Henshall DC
J. Neurochem., 2010-07-30;115(1):92-101.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot -
Activation of the extrinsic caspase pathway in cultured cortical neurons requires p53-mediated down-regulation of the X-linked inhibitor of apoptosis protein to induce apoptosis.
Authors: Tun C, Guo W, Nguyen H, Yun B, Libby RT, Morrison RS, Garden GA
J. Neurochem., 2007-05-04;102(4):1206-19.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Proteolysis of HIP during apoptosis occurs within a region similar to the BID loop.
Authors: Caruso JA, Reiners JJ
Apoptosis, 2006-11-01;11(11):1877-85.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Western Blot
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Could only detect the pro form in my samples.
