Human/Mouse BID Antibody

Name: Human/Mouse BID Antibody
Brand: R&D Systems
SKU: AF860
Rating: 4 (3 reviews)

Catalog #: AF860
21 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF860
AF860-SP

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Product Details

Citations (21)

FAQs

Supplemental Products

Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse BID Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse BID, a fragment generated in cells treated with anti-Fas and the carboxyl‑terminal portion of recombinant BID generated by cleavage with Caspase 8 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse BID
Met1-Asp195
Accession # AAC71064

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunoprecipitation

4 µg/10⁶ cells

Jurkat human acute T cell leukemia cell line treated with anti-Fas, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BID by Western Blot. Western blot shows Jurkat human acute T cell leukemia cell line treated with apoptosis inducer anti-Fas for the indicated times. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BID at approximately 20 kDa (as indicated). For additional reference short (5 seconds, left panel) and long (60 seconds, right panel) exposures are shown. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western Blot

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Detection of Human and Mouse BID by Western Blot. Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BID at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human BID by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for BID at approximately 29 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BID

BID is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1). BID is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells BID is cytosolic. In response to Fas ligand or TNF, BID is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane (2, 3). Cleavage of BID by caspase-8 generates a new N-terminal that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target BID to the mitochondria (4). BID may then interact with another pro-apoptotic Bcl-2 family member Bak (5). Interaction of BID with Bak causes altered mitochondrial membrane permeability. A 9‑13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the BID interaction with other Bcl-2 family members. BID is neutralized by binding to the anti-apoptotic member Bcl-xL.

References

Gross, A. et al. (1999) Genes and Develop. 13:1899.
Luo, X., et al. (1998) Cell 94:481.
Li, H. et al. (1998) Cell 94:491.
Zha, J. et al. (2000) Science 290:1761.
Wei, M.C. et al. (2000) Genes Dev. 14:2060.

Long Name

BH3 Interacting Domain Death Agonist

Entrez Gene IDs

637 (Human); 12122 (Mouse)

Alternate Names

apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID; tbid

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Human/Mouse BID Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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High Levels of Progesterone Receptor B in MCF-7 Cells Enable Radical Anti-Tumoral and Anti-Estrogenic Effect of Progestin
Authors: N Bajalovic, YZ Or, ARE Woo, SH Lee, VCL Lin
Biomedicines, 2022-08-02;10(8):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
p53-independent induction of p21 fails to control regeneration and hepatocarcinogenesis in a murine liver injury model
Authors: LE Buitrago-M, S Marhenke, D Becker, R Geffers, T Itzel, A Teufel, H Jaeschke, A Lechel, K Unger, J Markovic, AD Sharma, JU Marquardt, M Saborowski, A Saborowski, A Vogel
Cellular and Molecular Gastroenterology and Hepatology, 2021-01-21;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Plants expressing murine pro-apoptotic protein Bid do not have enhanced PCD
Authors: A Manara, Z Imanifard, L Fracasso, D Bellin, M Crimi
Bmc Research Notes, 2020-09-21;13(1):450.
Species: Arabidopsis thaliana
Sample Types: Cell Lysates
Applications: Western Blot
An Apoptotic Caspase Network Safeguards Cell Death Induction in Pyroptotic Macrophages
Authors: NM de Vasconc, N Van Opdenb, H Van Gorp, R Martín-Pér, A Zecchin, P Vandenabee, M Lamkanfi
Cell Rep, 2020-07-28;32(4):107959.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot
Phenotypic selection with an intrabody library reveals an anti-apoptotic function of PKM2 requiring Mitofusin-1
Authors: T Liu, T Kuwana, H Zhang, MG Vander Hei, RA Lerner, DD Newmeyer
PLoS Biol., 2019-06-10;17(6):e2004413.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Mitochondrial fusion and Bid-mediated mitochondrial apoptosis are perturbed by alcohol with distinct dependence on its metabolism
Authors: S Naghdi, WS Slovinsky, M Madesh, E Rubin, G Hajnóczky
Cell Death Dis, 2018-10-09;9(10):1028.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane rupture
Authors: NM de Vasconc, N Van Opdenb, H Van Gorp, E Parthoens, M Lamkanfi
Cell Death Differ., 2018-04-17;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Executioner caspases and CAD are essential for mutagenesis induced by TRAIL or vincristine
Authors: MA Miles, CJ Hawkins
Cell Death Dis, 2017-10-05;8(10):e3062.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Highly tumorigenic hepatocellular carcinoma cell line with cancer stem cell-like properties
Authors: B Lacoste, VA Raymond, S Cassim, P Lapierre, M Bilodeau
PLoS ONE, 2017-02-02;12(2):e0171215.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis.
Authors: Rizvi S, Mertens J, Bronk S, Hirsova P, Dai H, Roberts L, Kaufmann S, Gores G
J Biol Chem, 2014-06-27;289(33):22835-49.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Germline quality control: eEF2K stands guard to eliminate defective oocytes.
Authors: Chu H, Liao Y, Novak J, Hu Z, Merkin J, Shymkiv Y, Braeckman B, Dorovkov M, Nguyen A, Clifford P, Nagele R, Harrison D, Ellis R, Ryazanov A
Dev Cell, 2014-02-27;28(5):561-72.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

GLI3-dependent repression of DR4 mediates hedgehog antagonism of TRAIL-induced apoptosis.
Authors: Kurita S, Mott JL, Almada LL
Oncogene, 2010-06-21;29(34):4848-58.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Down-regulation of FoxO-dependent c-FLIP expression mediates TRAIL-induced apoptosis in activated hepatic stellate cells.
Authors: Park SJ, Sohn HY, Yoon J, Park SI
Cell. Signal., 2009-05-24;21(10):1495-503.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Sensitization of imatinib-resistant CML cells to TRAIL-induced apoptosis is mediated through down-regulation of Bcr-Abl as well as c-FLIP.
Authors: Park SJ, Kim MJ, Kim HB, Kang CD, Kim SH
Biochem. J., 2009-04-28;420(1):73-81.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Cardioprotection against myocardial infarction with PTD-BIR3/RING, a XIAP mimicking protein.
Authors: Souktani R, Pons S, Guegan C, Bouhidel O, Bruneval P, Zini R, Mandet C, Onteniente B, Berdeaux A, Ghaleh B
J. Mol. Cell. Cardiol., 2009-02-20;46(5):713-8.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Trichostatin A sensitizes human ovarian cancer cells to TRAIL-induced apoptosis by down-regulation of c-FLIPL via inhibition of EGFR pathway.
Authors: Park SJ, Kim MJ, Kim HB, Sohn HY, Bae JH, Kang CD, Kim SH
Biochem. Pharmacol., 2009-01-24;77(8):1328-36.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Yersinia YopP-induced apoptotic cell death in murine dendritic cells is partially independent from action of caspases and exhibits necrosis-like features.
Authors: Grobner S, Autenrieth SE, Soldanova I, Gunst DS, Schaller M, Bohn E, Muller S, Leverkus M, Wesselborg S, Autenrieth IB, Borgmann S
Apoptosis, 2006-11-01;11(11):1959-68.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot

Translocation of full-length Bid to mitochondria during anoikis.
Authors: Valentijn AJ, Gilmore AP
J. Biol. Chem., 2004-05-17;279(31):32848-57.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot

Early events in the anoikis program occur in the absence of caspase activation.
Authors: Wang P, Valentijn AJ, Gilmore AP, Streuli CH
J. Biol. Chem., 2003-03-05;278(22):19917-25.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Post-translational modification of Bid has differential effects on its susceptibility to cleavage by caspase 8 or caspase 3.
Authors: Degli Esposti M, Ferry G, Masdehors P, Boutin JA, Hickman JA, Dive C
J. Biol. Chem., 2003-02-21;278(18):15749-57.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
Authors: Jayanthi S, åÏctÐs, Deng X, Bordelon M, McCoy MT, Cadet JL
2001-08-01;15(10):1745-52.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot