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Human/Mouse Brachyury Antibody

Catalog #: AF2085
145 Citations 2 Reviews Datasheet / COA / SDS
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AF2085
AF2085-SP
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Detection of Recombinant Human Brachyury by Western Blot.
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Citations (145)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Brachyury Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Brachyury in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Brachyury
Ser2-Glu202
Accession # O15178
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1-1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
NCI-H460 cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human Brachyury antibody by Western Blot. View Larger

Detection of Recombinant Human Brachyury by Western Blot. Western blot shows 10 ng of Recombinant Human Brachyury (amino acids Ser2-Glu202) and Recombinant Human TBX6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Brachyury at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Western Blot View Larger

Detection of Human Brachyury by Western Blot. Western blot shows lysates of NCI‑H460 human large cell lung carcinoma cell line and MCF 10A human breast epithelial cell line (negative control). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Brachyury at approximately 50 kDa (as indicated). GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry Brachyury antibody in Differentiated Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

Brachyury in Differentiated Human Embryonic Stem Cells. Brachyury was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) for 3 hours at room temperature. Cells were stained (green) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Brachyury antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

Brachyury in BG01V Human Stem Cells. Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counter-stained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Brachyury antibody in Embryonic Mouse Notochord by Immunohistochemistry (IHC-Fr). View Larger

Brachyury in Embryonic Mouse Notochord. Brachyury was detected in immersion fixed frozen sections of embryonic mouse notochord (E9.5) using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) overnight at 4 °C. Tissue was stained with the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Chromatin Immunoprecipitation (ChIP) Detection of Brachyury-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of Brachyury-regulated Genes by Chromatin Immunoprecipitation. Mesoderm-differentiated BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Brachyury/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. TheVEGFpromoter was detected by standard PCR.

Intracellular Staining by Flow Cytometry View Larger

Detection of Brachyury in NCI-H460 cells by Flow Cytometry. NCI-H460 cells were stained with Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Brachyury

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Entrez Gene IDs
6862 (Human)
Alternate Names
brachyury protein; Brachyury; MGC104817; Protein T; SAVA; T brachyury (mouse) homolog; T brachyury homolog; T Brachyury Transcription Factor; T, brachyury homolog (mouse); T-Box Transcription Factor T; TBXT; TF; TFT

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Product Specific Notices

BG01V cells are licensed from ViaCyte, Inc.

Citations for Human/Mouse Brachyury Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

145 Citations: Showing 1 - 10
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  1. Establishing a human-induced pluripotent stem cell line (SMUSHi003-A) from a patient with Charcot-Marie-Tooth disease and focal segmental glomerulosclerosis
    Authors: Lei, Q;Zhou, W;Huang, L;Zhang, Y;Xu, X;Guo, X;
    Stem cell research
    Species: Human hepegivirus
    Sample Types: Organoid
    Applications: Immunocytochemistry
  2. Generation of hiPSCs (JUCGRMi003-A) from a patient with Parkinson's disease with PARK2 mutation
    Authors: Ishikawa, KI;Okuzumi, A;Yoshino, H;Hattori, N;Akamatsu, W;
    Stem cell research
    Species: Human hepegivirus
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  3. Generation and characterization of a human iPSC line (JUFMDOi007-A) from a patient with Usher syndrome due to mutation in USH2A
    Authors: T Ukaji, M Takahashi-, D Arai, H Tsutsumi, S Tajima, W Akamatsu, F Matsumoto, K Ikeda, SI Usami, K Kamiya
    Stem Cell Research, 2023-04-17;69(0):103100.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Generation of two induced pluripotent stem cell lines from spinal muscular atrophy type 1 patients carrying no functional copies of SMN1 gene
    Authors: W Zeng, X Kong, C Alamana, Y Liu, J Guzman, PD Pang, JW Day, JC Wu
    Stem Cell Research, 2023-04-17;69(0):103095.
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: ICC
  5. Generation of induced pluripotent stem cell line (UKWNLi008) derived from a patient carrying a c.1678C>G variant in the transient receptor potential cation channel subfamily A member (TRPA1) gene potentially associated with small fiber neuropathy
    Authors: NM Schottmann, K Klug, E Klopocki, N Üçeyler
    Stem Cell Research, 2023-04-12;69(0):103094.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: ICC
  6. Single-cell analysis of bidirectional reprogramming between early embryonic states reveals mechanisms of differential lineage plasticities
    Authors: V Garg, Y Yang, S Nowotschin, M Setty, YY Kuo, R Sharma, A Polyzos, E Salataj, D Murphy, A Jang, D Pe'er, E Apostolou, AK Hadjantona
    bioRxiv : the preprint server for biology, 2023-03-29;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ChIP, Flow Cytometry, ICC
  7. Generation of three induced Pluripotent Stem Cell lines from individuals with Hypomyelination with Atrophy of Basal Ganglia and Cerebellum caused by a c.745G>A (p.D249N) autosomal dominant mutation in TUBB4A
    Authors: AA Almad, L Garcia, A Takanohash, A Gagne, W Yang, J Ann McGuir, D French, A Vanderver
    Stem Cell Research, 2023-03-26;69(0):103083.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  8. Establishment of TUBB3-mCherry knock-in human pluripotent stem cell line using CRISPR/Cas9 (SNUe003-A-4)
    Authors: AH Kim, HM Lee, HS Kim, J Jung, H Seol, E Choi, S Lee, Y Min Choi, JK Jun, HS Kim, J Jang
    Stem Cell Research, 2023-03-08;69(0):103064.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  9. hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease
    Authors: Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer
    Cell Stem Cell, 2023-03-02;30(3):264-282.e9.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC
  10. Epigenetic and transcriptional regulations prime cell fate before division during human pluripotent stem cell differentiation
    Authors: P Madrigal, S Deng, Y Feng, S Militi, KJ Goh, R Nibhani, R Grandy, A Osnato, D Ortmann, S Brown, S Pauklin
    Nature Communications, 2023-01-25;14(1):405.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Generation of two induced pluripotent stem cell lines UKWNLi006 and UKWNLi007 derived from two patients with an active site GLA mutation leading to a pain and no pain phenotype in Fabry disease
    Authors: K Klug, M Breyer, E Klopocki, N Üçeyler
    Stem Cell Research, 2023-01-09;67(0):103025.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  12. hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease
    Authors: Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer
    Cell Stem Cell, 2023;30(3):264-282.e9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Generation of a human iPSC line (CIBi014-A) from a patient with Parkinson's disease carrying a novel heterozygotic PARK8 (LRRK2) mutation
    Authors: L Li, X Si, J Yang, M Lei, H Liu, J Ruan, H Fu, W Li, H Yang, X Lei, H Sang
    Stem Cell Research, 2022-12-09;66(0):102995.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. A reference human induced pluripotent stem cell line for large-scale collaborative studies
    Authors: CB Pantazis, A Yang, E Lara, JA McDonough, C Blauwendra, L Peng, H Oguro, J Kanaujiya, J Zou, D Sebesta, G Pratt, E Cross, J Blockwick, P Buxton, L Kinner-Bib, C Medura, C Tompkins, S Hughes, M Santiana, F Faghri, MA Nalls, D Vitale, S Ballard, YA Qi, DM Ramos, KM Anderson, J Stadler, P Narayan, J Papademetr, L Reilly, MP Nelson, S Aggarwal, LU Rosen, P Kirwan, V Pisupati, SL Coon, SW Scholz, T Priebe, M Öttl, J Dong, M Meijer, LJM Janssen, VS Lourenco, R van der Ka, D Crusius, D Paquet, AC Raulin, G Bu, A Held, BJ Wainger, RMC Gabriele, JM Casey, S Wray, D Abu-Bonsra, CL Parish, MS Beccari, DW Cleveland, E Li, IVL Rose, M Kampmann, C Calatayud, P Verstreken, L Heinrich, MY Chen, B Schüle, D Dou, ELF Holzbaur, MC Zanellati, R Basundra, M Deshmukh, S Cohen, R Khanna, M Raman, ZS Nevin, M Matia, J Van Lent, V Timmerman, BR Conklin, K Johnson Ch, K Zhang, S Funes, DA Bosco, L Erlebach, M Welzer, D Kronenberg, G Lyu, E Arenas, E Coccia, L Sarrafha, T Ahfeldt, JC Marioni, WC Skarnes, MR Cookson, ME Ward, FT Merkle
    Cell Stem Cell, 2022-12-01;29(12):1685-1702.e22.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: ICC
  15. Generation of induced pluripotent stem cells from three individuals with Huntington's disease
    Authors: DC Miller, P Lisowski, S Lickfett, B Mlody, M Bünning, C Genehr, C Ulrich, EE Wanker, S Diecke, J Priller, A Prigione
    Stem Cell Research, 2022-11-17;65(0):102976.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  16. Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension
    Authors: M Bao, J Cornwall-S, E Sanchez-Va, DY Chen, J De Jonghe, S Shadkhoo, F Hollfelder, M Thomson, DM Glover, M Zernicka-G
    Nature Cell Biology, 2022-09-13;24(9):1341-1349.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. LRP1 is a neuronal receptor for alpha-synuclein uptake and spread
    Authors: K Chen, YA Martens, A Meneses, DH Ryu, W Lu, AC Raulin, F Li, J Zhao, Y Chen, Y Jin, C Linares, M Goodwin, Y Li, CC Liu, T Kanekiyo, DM Holtzman, TE Golde, G Bu, N Zhao
    Molecular Neurodegeneration, 2022-09-02;17(1):57.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Spatial molecular anatomy of germ layers in the gastrulating cynomolgus monkey embryo
    Authors: G Cui, S Feng, Y Yan, L Wang, X He, X Li, Y Duan, J Chen, K Tang, P Zheng, PPL Tam, W Si, N Jing, G Peng
    Cell Reports, 2022-08-30;40(9):111285.
    Species: Cynomolgus Monkey
    Sample Types: Whole Cells
    Applications: ICC/IF
  19. Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS)
    Authors: J Contreras, M Alonzo, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao
    Stem Cell Research, 2022-08-10;64(0):102893.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  20. Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)
    Authors: M Alonzo, J Contreras, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao
    Stem Cell Research, 2022-08-09;64(0):102892.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  21. Production of a human iPSC line from an early-onset Parkinson's disease patient with a novel CHCHD2 gene truncated mutation
    Authors: Z Jiang, XJ Gu, WM Su, QQ Duan, JY Lin, B Cao, HF Shang, YP Chen
    Stem Cell Research, 2022-08-04;64(0):102881.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  22. Generation of a human induced pluripotent stem cell line carrying the TYR c.575C>A (p.Ser192Tyr) and c.1205G>A (p.Arg402Gln) variants in homozygous state using CRISPR-Cas9 genome editing
    Authors: J Liu, GC Black, SJ Kimber, PI Sergouniot
    Stem Cell Research, 2022-07-30;64(0):102880.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  23. In�vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion
    Authors: S Gupta, R Kawaguchi, E Heinrichs, S Gallardo, S Castellano, I Mandric, BG Novitch, SJ Butler
    Oncogene, 2022-07-19;40(3):111119.
    Species: Human
    Sample Types: Embryoid Bodies
    Applications: IHC
  24. Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1
    Authors: YH Chen, PS Huang, MH Wen, M Pan, DF Lee, TY Chen
    Oncogene, 2022-06-14;63(0):102845.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. A gastruloid model of the interaction between embryonic and extra-embryonic cell types
    Authors: NM Bérenger-C, M Mircea, E Adegeest, PR van den Be, M Feliksik, M Hochane, T Idema, SJ Tans, S Semrau
    Journal of tissue engineering, 2022-06-11;13(0):2041731422110.
    Species: Mouse
    Sample Types: Organoids
    Applications: IHC
  26. In�vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells
    Authors: M Simunovic, ED Siggia, AH Brivanlou
    Cell Stem Cell, 2022-06-02;29(6):962-972.e4.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  27. Generation of a human iPSC line CIBi011-A from amniocytes of a healthy fetus
    Authors: Y Shi, S Wu, B Yang, B Yu, Y Yan, J Yang, M Chu, X Hu
    Stem Cell Research, 2022-05-04;62(0):102801.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  28. Generation of a human induced pluripotent stem cell line FMUPDCi001-A from a patient with mental retardation, autosomal recessive 36 (MRT36) carrying the variants c.219dupA and c.587C�>�T in ADAT3
    Authors: X Hu, S Xiong, X Zhou, L Sun
    Stem Cell Research, 2022-04-04;61(0):102777.
    Species: Human
    Sample Types: Spheroid
    Applications: ICC
  29. Generation of alphaMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting
    Authors: HM Lee, AH Kim, S Hwang, J Jung, H Seol, JJ Sung, SM Jeong, YM Choi, JK Jun, HS Kim, J Jang
    Stem Cell Research, 2022-04-04;61(0):102779.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: ICC
  30. Generation of two iPSC lines from hypertrophic cardiomyopathy patients carrying MYBPC3 and PRKAG2 variants
    Authors: A Manhas, JWS Jahng, CD Vera, SP Shenoy, JW Knowles, JC Wu
    Stem Cell Research, 2022-03-31;61(0):102774.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  31. Generation of the induced pluripotent stem cell line UKWNLi005-A derived from a patient with the GLA mutation c.376A�>�G of unknown pathogenicity in Fabry disease
    Authors: M Breyer, T Klein, K Klug, E Klopocki, N Üçeyler
    Stem Cell Research, 2022-03-14;61(0):102747.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. Generation of a MSX1 knockout human embryonic stem cell line using CRISPR/Cas9 technology
    Authors: W Chiu, A Li, T Wang, W Li, X Zhang
    Stem Cell Research, 2022-02-26;60(0):102729.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts
    Authors: S Chumchuen, C Satirapod, A Tangprasit, S Hongeng
    Stem Cell Research, 2022-01-19;60(0):102675.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  34. Generation of iPSC line (FAMRCi009-A) from patient with familial progressive cardiac conduction disorder carrying genetic variant FLNC p.Val2264Met
    Authors: N Rodina, A Khudiakov, K Perepelina, A Muravyev, A Boytsov, A Zlotina, P Sokolnikov, A Kostareva
    Stem Cell Research, 2021-12-27;59(0):102640.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  35. Derivation and characterization of the induced pluripotent stem cell line CUIMCi004-A from a patient with a novel frameshift variant in exon 18a of OCRL
    Authors: G Iannello, A Patel, D Sirabella, B Corneo, VV Thaker
    Stem Cell Research, 2021-12-23;59(0):102635.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  36. Generation of iPSC line FAMRCi010-A from patient with restrictive cardiomyopathy carrying genetic variant FLNC p.Gly2011Arg
    Authors: K Perepelina, A Khudiakov, N Rodina, A Boytsov, T Vavilova, A Zlotina, P Sokolnikov, A Kostareva
    Stem Cell Research, 2021-12-21;59(0):102639.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  37. Generation of three iPSC lines from dilated cardiomyopathy patients carrying a pathogenic LMNA variant
    Authors: C Lee, S Cho, C Lai, S Shenoy, R Vagelos, JC Wu
    Stem Cell Research, 2021-12-21;59(0):102638.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  38. Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations
    Authors: N Belbachir, C Lai, JW Rhee, Y Zhuge, MV Perez, K Sallam, JC Wu
    Stem Cell Research, 2021-11-23;57(0):102605.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  39. StemBond hydrogels control the mechanical microenvironment for pluripotent stem cells
    Authors: C Labouesse, BX Tan, CC Agley, M Hofer, AK Winkel, GG Stirparo, HT Stuart, CM Verstreken, C Mulas, W Mansfield, P Bertone, K Franze, JCR Silva, KJ Chalut
    Nature Communications, 2021-10-21;12(1):6132.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  40. Comparison of Differentiation Pattern and WNT/SHH Signaling in Pluripotent Stem Cells Cultured under Different Conditions
    Authors: B ?wierczek-, D Dudka, D Bauer, T Czajkowski, K Ilach, W Streminska, A Kominek, K Piwocka, MA Ciemerych, K Archacka
    Cells, 2021-10-14;10(10):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Immunolocalization
  41. Uncovering the Metabolic and Stress Responses of Human Embryonic Stem Cells to FTH1 Gene Silencing
    Authors: L Scaramuzzi, V Lucchino, S Scalise, M Lo Conte, C Zannino, A Sacco, F Biamonte, EI Parrotta, FS Costanzo, G Cuda
    Cells, 2021-09-15;10(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. Rapid target validation in a Cas9-inducible hiPSC derived kidney model
    Authors: Y Shamshirga, A Jonebring, A Svensson, I Leefa, M Bohlooly-Y, M Firth, KJ Woollard, A Hofherr, IM Rogers, R Hicks
    Scientific Reports, 2021-08-16;11(1):16532.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  43. Global Transcriptional Analyses of the Wnt-Induced Development of Neural Stem Cells from Human Pluripotent Stem Cells
    Authors: BC Liu, FY Liu, XY Gao, YL Chen, QQ Meng, YL Song, XH Li, SQ Bao
    International Journal of Molecular Sciences, 2021-07-12;22(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  44. Dynamics of primitive streak regression controls the fate of neuromesodermal progenitors in the chicken embryo
    Authors: C Guillot, Y Djeffal, A Michaut, B Rabe, O Pourquié
    Elife, 2021-07-06;10(0):.
    Species: Chicken
    Sample Types: Embryo
    Applications: IHC
  45. Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity
    Authors: G Iannello, A Patel, D Sirabella, B Corneo, V Thaker
    Stem Cell Research, 2021-06-21;54(0):102432.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  46. Human iPSC lines from a Christianson syndrome patient with NHE6 W523X mutation, a biologically-related control, and CRISPR/Cas9 gene-corrected isogenic controls
    Authors: L Ma, M Schmidt, EM Morrow
    Stem Cell Research, 2021-06-18;54(0):102435.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  47. Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1
    Authors: PS Huang, MH Wen, X Xie, A Xu, DF Lee, TY Chen
    Stem Cell Research, 2021-06-06;54(0):102415.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  48. Establishment of an arrhythmogenic right ventricular cardiomyopathy derived iPSC cell line (USFi004-A) carrying a heterozygous mutation in PKP2 (c.1799delA)
    Authors: J Yang, E Samal, M Burgos Ang, A Bertalovit, TV McDonald
    Stem Cell Research, 2021-05-19;54(0):102398.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  49. Generation of an iPSC cell line (USFi003-A) from a patient with dilated cardiomyopathy carrying a heterozygous mutation in LMNA (p.R541C)
    Authors: J Yang, M Burgos Ang, MA Argenziano, A Bertalovit, M Najari Bei, TV McDonald
    Stem Cell Research, 2021-05-13;54(0):102396.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  50. Generation of a heterozygous FLNC mutation-carrying human iPSC line, USFi002-A, for modeling dilated cardiomyopathy
    Authors: MA Argenziano, M Burgos Ang, M Najari Bei, J Yang, AC Bertalovit, TV McDonald
    Stem Cell Research, 2021-05-12;53(0):102394.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  51. Induced pluripotent stem cells from subjects with Lesch-Nyhan disease
    Authors: DJ Sutcliffe, AR Dinasarapu, JE Visser, JD Hoed, F Seifar, P Joshi, I Ceballos-P, T Sardar, EJ Hess, YV Sun, Z Wen, ME Zwick, HA Jinnah
    Scientific Reports, 2021-04-19;11(1):8523.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  52. Generation of human induced pluripotent stem cell lines (UNIMGi003-A and UNIMGi004-A) from two Italian siblings affected by Unverricht-Lundborg disease
    Authors: V Lucchino, L Scaramuzzi, S Scalise, K Grillone, M Lo Conte, C Esposito, U Aguglia, E Ferlazzo, N Perrotti, P Malatesta, EI Parrotta, G Cuda
    Stem Cell Research, 2021-04-09;53(0):102329.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  53. Generation of pathogenic TPP1 mutations in human stem cells as a model for neuronal ceroid lipofuscinosis type 2 disease
    Authors: L Ma, AM Prada, M Schmidt, EM Morrow
    Stem Cell Research, 2021-04-06;53(0):102323.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  54. Generation of an iPSC line (CRICKi001-A) from an individual with a germline SMARCA4 missense mutation and autism spectrum disorder
    Authors: LG Devito, L Healy, S Mohammed, F Guillemot, C Dias
    Stem Cell Research, 2021-03-20;53(0):102304.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  55. Generation of three induced pluripotent stem cell lines, SCVIi003-A, SCVIi004-A, SCVIi005-A, from patients with ARVD/C caused by heterozygous mutations in the PKP2 gene
    Authors: JWS Jahng, KE Black, L Liu, HR Bae, M Perez, EA Ashley, K Sallam, JC Wu
    Stem Cell Research, 2021-03-12;53(0):102284.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  56. Generation of two heterozygous MYBPC3 mutation-carrying human iPSC lines, SCVIi001-A and SCVIi002-A, for modeling hypertrophic cardiomyopathy
    Authors: L Liu, SP Shenoy, JWS Jahng, Y Liu, JW Knowles, Y Zhuge, JC Wu
    Stem Cell Research, 2021-03-11;53(0):102279.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  57. Generation of a set of isogenic iPSC lines carrying all APOE genetic variants (?2/?3/?4) and knock-out for the study of APOE biology in health and disease
    Authors: B Schmid, B Holst, C Clausen, L Bahnassawy, P Reinhardt, MHM Bakker, E Díaz-Guerr, C Vicario, PV Martino-Ad, M Thoenes, A Ramirez, K Flissbach, C Grezella, O Brüstle, M Peitz, A Ebneth, A Cabrera-So
    Stem Cell Research, 2021-02-02;52(0):102180.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  58. Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency
    Authors: M Kinoshita, M Barber, W Mansfield, Y Cui, D Spindlow, GG Stirparo, S Dietmann, J Nichols, A Smith
    Cell Stem Cell, 2020-12-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  59. Generation of iPSC lines from two patients affected by febrile seizure due to inherited missense mutation in SCN1A gene
    Authors: S Scalise, L Scaramuzzi, V Lucchino, C Esposito, P Malatesta, K Grillone, N Perrotti, G Cuda, EI Parrotta
    Stem Cell Research, 2020-11-07;49(0):102083.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  60. Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells
    Authors: FJ Hawkins, S Suzuki, ML Beermann, C Barillà, R Wang, C Villacorta, A Berical, JC Jean, J Le Suer, T Matte, C Simone-Roa, Y Tang, TM Schlaeger, AM Crane, N Matthias, SXL Huang, SH Randell, J Wu, JR Spence, G Carraro, BR Stripp, A Rab, EJ Sorsher, A Horani, SL Brody, BR Davis, DN Kotton
    Cell Stem Cell, 2020-10-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  61. Molecular Analysis of Fetal and Adult Primary Human Liver Sinusoidal Endothelial Cells: A Comparison to Other Endothelial Cells
    Authors: MA Jamil, H Singer, R Al-Rifai, N Nüsgen, M Rath, S Strauss, I Andreou, J Oldenburg, O El-Maarri
    Int J Mol Sci, 2020-10-21;21(20):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  62. Generation of 10 patient-specific induced pluripotent stem cells (iPSCs) to model Pitt-Hopkins Syndrome
    Authors: SR Sripathy, Y Wang, RL Moses, A Fatemi, DA Batista, BJ Maher
    Stem Cell Res, 2020-09-17;48(0):102001.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  63. Generation of two iPS cell lines (HIHDNDi001-A and HIHDNDi001-B) from a Parkinson�s disease patient carrying the heterozygous p.A30P mutation in SNCA
    Authors: PA Barbuti, BFR Santos, CM Dording, G Cruciani, F Massart, A Hummel, R Krüger
    Stem Cell Res, 2020-08-08;48(0):101951.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  64. Generation of two iPSC lines (FAMRCi007-A and FAMRCi007-B) from patient with Emery-Dreifuss muscular dystrophy and heart rhythm abnormalities carrying genetic variant LMNA p.Arg249Gln
    Authors: K Perepelina, A Kostina, P Klauzen, A Khudiakov, M Rabino, S Crasto, A Zlotina, Y Fomicheva, A Sergushich, M Oganesian, A Dmitriev, A Kostareva, E Di Pasqual, A Malashiche
    Stem Cell Res, 2020-06-29;47(0):101895.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  65. Generation and characterization of four Chediak-Higashi Syndrome (CHS) induced pluripotent stem cell (iPSC) lines
    Authors: J Serra-Vina, MB Sandler, E Pak, W Zheng, A Dutra, W Introne, WA Gahl, MC Malicdan
    Stem Cell Res, 2020-06-22;47(0):101883.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  66. Crisponi syndrome/cold-induced sweating syndrome type 2: Reprogramming of CS/CISS2 individual derived fibroblasts into three clones of one iPSC line
    Authors: I Buers, L Schöning, N Tomas Loge, Y Nitschke, IM Höben, A Röpke, L Crisponi, H Omran, F Rutsch
    Stem Cell Res, 2020-06-01;46(0):101855.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  67. Generation of induced pluripotent stem cell lines from a Crisponi/Cold induced sweating syndrome type 1 individual
    Authors: L Schöning, NT Loges, Y Nitschke, IM Höben, A Röpke, L Crisponi, H Omran, F Rutsch, I Buers
    Stem Cell Res, 2020-05-28;46(0):101820.
    Species: Human
    Sample Types: Whole Cell
    Applications: Flow Cytometry, ICC
  68. GATA3 Mediates a Fast, Irreversible Commitment to BMP4-Driven Differentiation in Human Embryonic Stem Cells
    Authors: A Gunne-Brad, A Sullivan, B Gharibi, RSM Sheriff, A Maity, YF Wang, A Edwards, M Jiang, M Howell, R Goldstone, R Wollman, P East, SDM Santos
    Cell Stem Cell, 2020-04-16;26(5):693-706.e9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  69. Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis
    Authors: AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen
    Cell Rep, 2020-04-07;31(1):107471.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  70. Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation
    Authors: M Chen, M Maimaitili, SH Buchholdt, UB Jensen, F Febbraro, M Denham
    Stem Cell Res, 2020-03-29;45(0):101781.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  71. Generation of two induced pluripotent stem cell lines (FAMRCi005-A and FAMRCi005-B) from patient carrying genetic variant LMNA p.Asp357Val
    Authors: P Klauzen, K Perepelina, A Khudiakov, A Zlotina, Y Fomicheva, T Pervunina, T Vershinina, A Kostareva, A Malashiche
    Stem Cell Res, 2020-02-05;43(0):101719.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  72. Generation of two iPSC lines (FAMRCi004-A and FAMRCi004-B) from patient with familial progressive cardiac conduction disorder carrying genetic variant DSP p.His1684Arg
    Authors: A Khudiakov, K Perepelina, P Klauzen, A Zlotina, K Gusev, E Kaznacheye, A Malashiche, A Kostareva
    Stem Cell Res, 2020-02-04;43(0):101720.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IF/ICC
  73. Generation of two iPSC lines (FAMRCi006-A and FAMRCi006-B) from patient with dilated cardiomyopathy and Emery-Dreifuss muscular dystrophy associated with genetic variant LMNAp.Arg527Pro
    Authors: K Perepelina, P Klauzen, A Khudiakov, A Zlotina, Y Fomicheva, D Rudenko, M Gordeev, A Sergushich, A Malashiche, A Kostareva
    Stem Cell Res, 2020-01-31;43(0):101714.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  74. Chromosome Transplantation: A Possible Approach to Treat Human X-linked Disorders
    Authors: M Paulis, L Susani, A Castelli, T Suzuki, T Hara, L Straniero, S Duga, D Strina, S Mantero, E Caldana, LS Sergi, A Villa, P Vezzoni
    Mol Ther Methods Clin Dev, 2020-01-21;17(0):369-377.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  75. In Vitro Fabrication of Hybrid Bone/Cartilage Complex Using Mouse Induced Pluripotent Stem Cells
    Authors: P Limraksasi, T Kondo, M Zhang, H Okawa, T Osathanon, P Pavasant, H Egusa
    Int J Mol Sci, 2020-01-16;21(2):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  76. Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings
    Authors: Y Hu, CM Domínguez, J Bauer, S Weigel, A Schipperge, C Oelschlaeg, N Willenbach, S Keppler, M Bastmeyer, S Hei beta ler, C Wöll, T Scharnwebe, KS Rabe, CM Niemeyer
    Nat Commun, 2019-12-04;10(1):5522.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  77. Induced pluripotent stem cell line (PEIi003-A) derived from an apparently healthy male individual
    Authors: NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, D Steinemann, G Göhring, R König
    Stem Cell Res, 2019-12-04;42(0):101679.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  78. Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells
    Authors: P Wörsdörfer, N Dalda, A Kern, S Krüger, N Wagner, CK Kwok, E Henke, S Ergün
    Sci Rep, 2019-10-30;9(1):15663.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  79. Induced pluripotent stem cell line (INSAi001-A) from a Gaucher disease type 3 patient compound heterozygote for mutations in the GBA1 gene
    Authors: AJ Duarte, D Ribeiro, R Santos, L Moreira, J Bragança, O Amaral
    Stem Cell Res, 2019-10-18;41(0):101595.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  80. Induced pluripotent stem cells (iPSCs) derived from a renpenning syndrome patient with c.459_462delAGAG mutation in PQBP1 (PEIi001-A)
    Authors: NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, V des Portes, D Germanaud, D Steinemann, G Göhring, R König
    Stem Cell Res, 2019-10-15;41(0):101592.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  81. Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids
    Authors: S Chhabra, L Liu, R Goh, X Kong, A Warmflash
    PLoS Biol., 2019-10-15;17(10):e3000498.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  82. Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus
    Authors: MT De Angelis, G Santamaria, EI Parrotta, S Scalise, M Lo Conte, S Gasparini, E Ferlazzo, U Aguglia, C Ciampi, A Sgura, G Cuda
    J. Cell. Mol. Med., 2019-09-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  83. Generation of an induced pluripotent stem cell line (FRIMOi007-A) derived from an incomplete achromatopsia patient carrying a novel homozygous mutation in PDE6C gene
    Authors: J Domingo-Pr, V Abad-Moral, M Riera, R Navarro, B Corcostegu, E Pomares
    Stem Cell Res, 2019-09-04;40(0):101569.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  84. Generation of Best disease-derived induced pluripotent stem cell line (FRIMOi006-A) carrying a novel dominant mutation in BEST1 gene
    Authors: J Domingo-Pr, M Riera, V Abad-Moral, S Ruiz-Nogal, B Corcostegu, E Pomares
    Stem Cell Res, 2019-09-04;40(0):101570.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  85. Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23)
    Authors: S Park, JE Yoo, DR Lee, J Jang, MS Cho, DS Kim, DW Kim
    Stem Cell Res, 2019-07-18;39(0):101499.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  86. Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis
    Authors: M Huang, J Tailor, Q Zhen, AH Gillmor, ML Miller, H Weishaupt, J Chen, T Zheng, EK Nash, LK McHenry, Z An, F Ye, Y Takashima, J Clarke, H Ayetey, FMG Cavalli, B Luu, BS Moriarity, S Ilkhanizad, L Chavez, C Yu, KM Kurian, T Magnaldo, N Sevenet, P Koch, SM Pollard, P Dirks, MP Snyder, DA Largaespad, YJ Cho, JJ Phillips, FJ Swartling, AS Morrissy, M Kool, SM Pfister, MD Taylor, A Smith, WA Weiss
    Cell Stem Cell, 2019-06-13;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  87. Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene
    Authors: J Domingo-Pr, M Riera, A Burés-Jels, B Corcostegu, E Pomares
    Stem Cell Res, 2019-05-22;38(0):101468.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  88. Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency
    Authors: T Kalkan, S Bornelöv, C Mulas, E Diamanti, T Lohoff, M Ralser, S Middelkamp, P Lombard, J Nichols, A Smith
    Cell Stem Cell, 2019-04-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  89. User-defined morphogen patterning for directing human cell fate stratification
    Authors: MC Regier, JJ Tokar, JW Warrick, L Pabon, E Berthier, DJ Beebe, KR Stevens
    Sci Rep, 2019-04-23;9(1):6433.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  90. Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells
    Authors: X Wei, J Guo, Q Li, Q Jia, Q Jing, Y Li, B Zhou, J Chen, S Gao, X Zhang, M Jia, C Niu, W Yang, X Zhi, X Wang, D Yu, L Bai, L Wang, J Na, Y Zou, J Zhang, S Zhang, D Meng
    Sci Adv, 2019-03-13;5(3):eaau7887.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  91. Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations
    Authors: M Riera, A Patel, A Burés-Jels, B Corcostegu, S Chang, E Pomares, B Corneo, JR Sparrow
    Stem Cell Res, 2019-02-13;36(0):101389.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  92. Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations
    Authors: M Riera, A Patel, B Corcostegu, S Chang, JR Sparrow, E Pomares, B Corneo
    Stem Cell Res, 2019-01-17;35(0):101385.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  93. Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene
    Authors: M Riera, A Patel, B Corcostegu, S Chang, B Corneo, JR Sparrow, E Pomares
    Stem Cell Res, 2019-01-17;35(0):101386.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  94. Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene
    Authors: JA Maguire, AL Gagne, P Gonzalez-A, BL Davidson, V Shakkottai, P Gadue, DL French
    Stem Cell Res, 2018-12-10;34(0):101361.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  95. Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation
    Authors: KE Worley, AS Chin, LQ Wan
    Stem Cells Int, 2018-12-02;2018(0):1848605.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  96. Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143.
    Authors: Baulier E, Garcia Diaz A, Corneo B, Farber D
    Stem Cell Res, 2018-11-28;33(0):274-277.
  97. Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel
    Authors: X Gao, JJ Yourick, RL Sprando
    Stem Cell Res, 2018-07-27;31(0):193-196.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  98. Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL
    Authors: AL Gagne, JA Maguire, S Gandre-Bab, ST Chou, SK Tasian, ML Loh, MJ Weiss, P Gadue, DL French
    Stem Cell Res, 2018-07-05;31(0):157-160.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  99. In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells
    Authors: SE Harrison, B Sozen, M Zernicka-G
    Nat Protoc, 2018-07-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  100. The non-canonical Wnt-PCP pathway shapes the caudal neural plate
    Authors: B López-Esco, JM Caro-Vega, DS Vijayragha, TF Plageman, JA Sanchez-Al, RC Moreno, D Savery, J Márquez-Ri, LA Davidson, P Ybot-Gonzá
    Development, 2018-05-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  101. The lipodystrophic hotspot lamin A p.R482W mutation deregulates the mesodermal inducer T/Brachyury and early vascular differentiation gene networks
    Authors: N Briand, AC Guénantin, D Jeziorowsk, A Shah, M Mantecon, E Capel, M Garcia, A Oldenburg, J Paulsen, JS Hulot, C Vigouroux, P Collas
    Hum. Mol. Genet., 2018-04-15;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  102. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
    Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
    Cell Stem Cell, 2018-04-05;22(4):543-558.e12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  103. ADAM17 is required for EGF-R-induced intestinal tumors via IL-6 trans-signaling
    Authors: S Schmidt, N Schumacher, J Schwarz, S Tangermann, L Kenner, M Schlederer, M Sibilia, M Linder, A Altendorf-, T Knösel, ES Gruber, G Oberhuber, J Bolik, A Rehman, A Sinha, J Lokau, P Arnold, AS Cabron, F Zunke, C Becker-Pau, A Preaudet, P Nguyen, J Huynh, S Afshar-Ste, AL Chand, J Westermann, PJ Dempsey, C Garbers, D Schmidt-Ar, P Rosenstiel, T Putoczki, M Ernst, S Rose-John
    J. Exp. Med., 2018-02-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  104. Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions
    Authors: A Yachie-Kin, K Onishi, J Ostblom, MA Langley, E Posfai, J Rossant, PW Zandstra
    Mol. Syst. Biol., 2018-01-29;14(1):e7952.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  105. Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells
    Authors: MT De Angelis, EI Parrotta, G Santamaria, G Cuda
    Cell Death Dis, 2018-01-05;9(1):6.
    Species: Human
    Sample Types: Embryoid Bodies
    Applications: ICC
  106. Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female
    Authors: S Martins, M Bohndorf, F Schröter, F Assar, W Wruck, K Sleegers, C Van Broeck, J Adjaye
    Stem Cell Res, 2017-12-13;26(0):76-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  107. The circular RNA circBIRC6 participates in the molecular circuitry controlling human pluripotency
    Authors: CY Yu, TC Li, YY Wu, CH Yeh, W Chiang, CY Chuang, HC Kuo
    Nat Commun, 2017-10-27;8(1):1149.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  108. In Vitro Micropatterned Human Pluripotent Stem Cell Test (�P-hPST) for Morphometric-Based Teratogen Screening
    Authors: J Xing, Y Cao, Y Yu, H Li, Z Song, H Yu
    Sci Rep, 2017-08-17;7(1):8491.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  109. CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells
    Authors: KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima
    Stem Cell Reports, 2017-06-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  110. Guided self-organization and cortical plate formation in human brain organoids
    Authors: MA Lancaster, NS Corsini, S Wolfinger, EH Gustafson, AW Phillips, TR Burkard, T Otani, FJ Livesey, JA Knoblich
    Nat. Biotechnol., 2017-05-31;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  111. A Gradient of Glycolytic Activity Coordinates FGF and Wnt Signaling during Elongation of the Body Axis in Amniote Embryos
    Authors: M Oginuma, P Moncuquet, F Xiong, E Karoly, J Chal, K Guevorkian, O Pourquié
    Dev. Cell, 2017-02-27;40(4):342-353.e10.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  112. The p53 Family Coordinates Wnt and Nodal Inputs in Mesendodermal Differentiation of Embryonic Stem Cells
    Authors: Joan Massagué
    Cell Stem Cell, 2016-11-23;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  113. Real-time and Non-invasive Monitoring of Embryonic Stem Cell Survival during the Development of Embryoid Bodies with Smart Nanosensor
    Authors: Dong-An Wang
    Acta Biomater, 2016-11-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  114. Jak1/Stat3 signaling acts as a positive regulator of pluripotency in chicken pre-gastrula embryos
    Authors: Kiyokazu Agata
    Dev. Biol., 2016-11-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  115. Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells
    PLoS ONE, 2016-11-01;11(11):e0165715.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  116. ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation
    Stem Cell Res, 2016-08-04;17(2):222-227.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  117. Visualization of the Epiblast and Visceral Endodermal Cells Using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells
    PLoS ONE, 2016-07-13;11(7):e0159246.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  118. Dynamic Heterogeneity of Brachyury in Mouse Epiblast Stem Cells Mediates Distinct Response to Extrinsic Bone Morphogenetic Protein (BMP) Signaling
    Authors: L Song, J Chen, G Peng, K Tang, N Jing
    J. Biol. Chem., 2016-05-16;291(29):15212-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  119. PI3K/mTORC2 regulates TGF-beta/Activin signalling by modulating Smad2/3 activity via linker phosphorylation.
    Authors: Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, Cui W
    Nat Commun, 2015-05-22;6(0):7212.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  120. An alternative pluripotent state confers interspecies chimaeric competency.
    Authors: Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause M, Nery J, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim N, Lajara J, Guillen P, Campistol J, Esteban C, Ross P, Saghatelian A, Ren B, Ecker J, Izpisua Belmonte J
    Nature, 2015-05-06;521(7552):316-21.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: IHC
  121. Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation.
    Authors: Garriock R, Chalamalasetty R, Kennedy M, Canizales L, Lewandoski M, Yamaguchi T
    Development, 2015-05-01;142(9):1628-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  122. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
    Authors: Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S
    Nat Biotechnol, 2014-11-10;33(1):89-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  123. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.
    Authors: Gouti, Mina, Tsakiridis, Anestis, Wymeersch, Filip J, Huang, Yali, Kleinjung, Jens, Wilson, Valerie, Briscoe, James
    PLoS Biol, 2014-08-26;12(8):e1001937.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  124. SRF is essential for mesodermal cell migration during elongation of the embryonic body axis.
    Authors: Schwartz B, Marks M, Wittler L, Werber M, Wahrisch S, Nordheim A, Herrmann B, Grote P
    Mech Dev, 2014-07-11;133(0):23-35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  125. T-box transcription factor brachyury is associated with prostate cancer progression and aggressiveness.
    Authors: Pinto F, Pertega-Gomes N, Pereira M, Vizcaino J, Monteiro P, Henrique R, Baltazar F, Andrade R, Reis R
    Clin Cancer Res, 2014-07-09;20(18):4949-61.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  126. Regulation of osteoclast homeostasis and inflammatory bone loss by MFG-E8.
    Authors: Abe T, Shin J, Hosur K, Udey M, Chavakis T, Hajishengallis G
    J Immunol, 2014-06-23;193(3):1383-91.
    Species: Mouse
    Sample Types: Whole Organism
    Applications: In Vivo
  127. Insulin-like growth factor promotes cardiac lineage induction in vitro by selective expansion of early mesoderm.
    Authors: Engels M, Rajarajan K, Feistritzer R, Sharma A, Nielsen U, Schalij M, de Vries A, Pijnappels D, Wu S
    Stem Cells, 2014-06-01;32(6):1493-502.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  128. Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm.
    Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, Nakamura M, Sutou K, Osafune K, Yamanaka S
    Nat Commun, 2014-04-24;5(0):3678.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  129. Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.
    Authors: Tsakiridis, Anestis, Huang, Yali, Blin, Guillaum, Skylaki, Stavroul, Wymeersch, Filip, Osorno, Rodrigo, Economou, Costas, Karagianni, Eleni, Zhao, Suling, Lowell, Sally, Wilson, Valerie
    Development, 2014-03-01;141(6):1209-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  130. The role of Hath6, a newly identified shear-stress-responsive transcription factor, in endothelial cell differentiation and function.
    Authors: Fang F, Wasserman S, Torres-Vazquez J, Weinstein B, Cao F, Li Z, Wilson K, Yue W, Wu J, Xie X, Pei X
    J Cell Sci, 2014-01-24;127(0):1428-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  131. Oct4 is required ~E7.5 for proliferation in the primitive streak.
    Authors: DeVeale B, Brokhman I, Mohseni P, Babak T, Yoon C, Lin A, Onishi K, Tomilin A, Pevny L, Zandstra P, Nagy A, van der Kooy D
    PLoS Genet, 2013-11-14;9(11):e1003957.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  132. L-Proline induces a mesenchymal-like invasive program in embryonic stem cells by remodeling H3K9 and H3K36 methylation.
    Authors: Comes, Stefania, Gagliardi, Miriam, Laprano, Nicola, Fico, Annalisa, Cimmino, Amelia, Palamidessi, Andrea, De Cesare, Dario, De Falco, Sandro, Angelini, Claudia, Scita, Giorgio, Patriarca, Eduardo, Matarazzo, Maria R, Minchiotti, Gabriell
    Stem Cell Reports, 2013-10-10;1(4):307-21.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  133. Genomic targets of Brachyury (T) in differentiating mouse embryonic stem cells.
    Authors: Evans AL, Faial T, Gilchrist MJ
    PLoS ONE, 2012-03-30;7(3):e33346.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  134. The endothelial cell line bEnd.3 maintains human pluripotent stem cells.
    Authors: Joubin, Katherin, Richardson, Amelia, Novoa, Natalia, Tu, Edmund, Tomishima, Mark J
    Stem Cells Dev, 2012-02-21;21(12):2312-21.
    Species: Human
    Sample Types: Whole Cells
  135. A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3.
    Authors: Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ
    J. Cell. Sci., 2011-05-24;124(0):1992-2000.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  136. A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells.
    Authors: Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P
    Dev. Biol., 2009-04-07;330(2):286-304.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  137. Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
    Authors: Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M
    Cell Stem Cell, 2009-03-06;4(3):248-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  138. TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal.
    Authors: Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL
    Nat. Cell Biol., 2008-06-22;10(7):837-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  139. Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells.
    Authors: Zhang P, Li J, Tan Z, Wang C, Liu T, Chen L, Yong J, Jiang W, Sun X, DU L, Ding M, Deng H
    Blood, 2007-11-27;111(4):1933-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  140. Creation of engineered human embryonic stem cell lines using phiC31 integrase.
    Authors: Thyagarajan B, Liu Y, Shin S, Lakshmipathy U, Scheyhing K, Xue H, Ellerstrom C, Strehl R, Hyllner J, Rao MS, Chesnut JD
    Stem Cells, 2007-10-25;26(1):119-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  141. Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
    Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
    Biotechnol. Bioeng., 2007-08-15;97(6):1617-25.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  142. A porous photocurable elastomer for cell encapsulation and culture.
    Authors: Gerecht S, Townsend SA, Pressler H, Zhu H, Nijst CL, Bruggeman JP, Nichol JW, Langer R
    Biomaterials, 2007-08-09;28(32):4826-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  143. Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed.
    Authors: McLean AB, D'Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S
    Stem Cells, 2007-01-01;25(1):29-38.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  144. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
    Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
    FEBS Lett., 2006-10-02;580(25):5869-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  145. Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.
    Authors: Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S
    Proc. Natl. Acad. Sci. U.S.A., 2006-04-21;103(18):6907-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human/Mouse Brachyury Antibody
By Leslie Priddy on 04/13/2018
Application: IHC Sample Tested: Tumor cell lyastes Species: Mouse
Human/Mouse Brachyury Antibody
By Anonymous on 09/12/2016
Application: ICC/IF Sample Tested: Embryonic stem cells,Mesodermal cells differentiated from mouse ESCs Species: Mouse

Immunohistochemistry on fixed 2D cell culture.
Primary antibody was used at 5ug/mL and incubated overnight. Brachyury-positive cells are visualized in red, nuclei are counterstained by Dapi (blue).

Immunocytochemistry/Immunofluorescence Human/Mouse Brachyury Antibody AF2085