Human/Mouse Brachyury APC-conjugated Antibody Summary
Ser2-Glu202
Accession # O15178
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085A, filled histogram) or isotype control antibody (IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
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Detection of Brachyury in IPSC mesoderm differentiation cells by Flow Cytometry. IPSC mesoderm differentiation cells treated with IPSC mesoderm differentiation (SC030B) for 2 days were stained with Goat Anti-Human/Mouse Brachyury APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with FC004 and permeabilized with FoxP3 Perm. View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Brachyury
Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.
Product Datasheets
Citations for Human/Mouse Brachyury APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease
Authors: Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer
Cell Stem Cell, 2023-03-02;30(3):264-282.e9.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease
Authors: Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer
Cell Stem Cell, 2023;30(3):264-282.e9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Lateral plate mesoderm cell-based organoid system for NK cell regeneration from human pluripotent stem cells
Authors: D Huang, J Li, F Hu, C Xia, Q Weng, T Wang, H Peng, B Wu, H Wu, J Xiong, Y Lin, Y Wang, Q Zhang, X Liu, L Liu, X Zheng, Y Geng, X Du, X Zhu, L Wang, J Hao, J Wang
Cell Discovery, 2022-11-08;8(1):121.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Microglia-like Cells Promote Neuronal Functions in Cerebral Organoids
Authors: I Fagerlund, A Dougalis, A Shakirzyan, M Gómez-Budi, A Pelkonen, H Konttinen, S Ohtonen, MF Fazaludeen, M Koskuvi, J Kuusisto, D Hernández, A Pebay, J Koistinaho, T Rauramaa, Š Lehtonen, P Korhonen, T Malm
Cells, 2021-12-30;11(1):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells
Authors: KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima
Stem Cell Reports, 2017-06-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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Human induced pluripotent stem cells were differentiated into mesodermal lineage cells for 4 days in culture. Cells were fixed, permeabilized, and stained with or without antibody for 45 minutes. Unstained and Brachyury-APC stained samples were run on a BD Accuri C6 flow cytometer for analysis.
