Human/Mouse Brachyury PE-conjugated Antibody Summary
Ser2-Glu202
Accession # O15178
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085P, filled histogram) or isotype control antibody (IC108P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
View Larger
Detection of Brachyury in NCI-H460 cells by Flow Cytometry. NCI-H460 cells were stained with Goat Anti-Human/Mouse Brachyury PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Brachyury
Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.
Product Datasheets
Citations for Human/Mouse Brachyury PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
4
Citations: Showing 1 - 4
Filter your results:
Filter by:
-
Stage-specific regulation of DNA methylation by TET enzymes during human cardiac differentiation
Authors: Y Lan, KM Banks, H Pan, N Verma, GR Dixon, T Zhou, B Ding, O Elemento, S Chen, D Huangfu, T Evans
Cell Reports, 2021-12-07;37(10):110095.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, ICC, Western Blot -
STRAP Deficiency Impairs Mouse Embryonic Stem Cells Lineage Commitment Through CYP26A1-Mediated Retinoic Acid Homeostasis
Authors: L Jin, C Chang, KM Pawlik, A Datta, LM Johnson, T Vu, JL Napoli, PK Datta
Stem Cells, 2018-07-17;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells
Authors: Annabelle Congras
Sci Rep, 2016-06-01;6(0):27059.
Species: Porcine
Sample Types: Whole Cells
Applications: Flow Cytometry -
Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
Authors: Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M
Cell Stem Cell, 2009-03-06;4(3):248-62.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse Brachyury PE-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse Brachyury PE-conjugated Antibody and earn rewards!
Have you used Human/Mouse Brachyury PE-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
